Muniesa P, Dominguez L
Departamento de Anatomia y Embriología y Genética Animal, Facultad de Veterinaria, Zaragoza, Spain.
Cell Differ Dev. 1990 Aug;31(2):105-17. doi: 10.1016/0922-3371(90)90014-n.
The spatial distribution, germ layer location, number and appearance of primordial germ cells (PGCs) are studied. Blastoderms from stage X (Eyal-Giladi and Kochav, 1976) to stage 2 (Hamburger and Hamilton, 1951) were serially sectioned and distribution patterns of PGCs were reconstructed. The presence of PGCs in clusters was noted at all stages examined. PGCs were found in relation to the epiblast, the primary hypoblast and in the blastocoel, during the whole period under examination. These observations show that PGCs can be directly studied at blastula stages. The results are consistent with observations on an early segregation of chick germ line that begins as early as stage X.
研究了原始生殖细胞（PGCs）的空间分布、胚层位置、数量和外观。对从X期（Eyal-Giladi和Kochav，1976）到2期（Hamburger和Hamilton，1951）的囊胚进行连续切片，并重建了PGCs的分布模式。在所有检查阶段均注意到PGCs以簇状存在。在整个检查期间，在与上胚层、初级下胚层以及囊胚腔相关的位置发现了PGCs。这些观察结果表明，在囊胚期可以直接研究PGCs。结果与早在X期就开始的鸡生殖系早期分离的观察结果一致。
