Eyal-Giladi H, Kochav S, Menashi M K
Differentiation. 1976 Mar 16;6(1):13-6. doi: 10.1111/j.1432-0436.1976.tb01462.x.
An attempt was made to re-examine the location of the primordial germ cells (PGCs) in very young chick embryos. Freshly laid blastoderms, prior to hypoblast formation, of a known anterio-posterior axis, were transversely bisected and each half was separately grown in vitro. Both anterior and posterior halves were shown to be fertile and each was shown to contain roughly the same amount of PGCs as a normal control embryo. It has been concluded that in the chick as well as in the duck there is no concentration of cells containing germinal plasm in the posterior part of the blastoderm. Two other possibilities should be investigated: 1. A concentric arrangement of cells containing germinal plasm. 2. The absence of a germinal plasm and a relatively late appearance of PGCs as a result of induction.
有人试图重新研究极幼龄鸡胚中原始生殖细胞（PGCs）的位置。在已知前后轴的情况下，将刚产下的、尚未形成下胚层的胚盘横向二等分，然后将每一半分别在体外培养。结果表明，前后两半都具有生殖能力，并且每一半所含的PGCs数量与正常对照胚胎大致相同。得出的结论是，在鸡和鸭中，胚盘后部都不存在含有生殖质的细胞聚集。另外两种可能性值得研究：1. 含有生殖质的细胞呈同心排列。2. 不存在生殖质，并且由于诱导作用PGCs出现相对较晚。
