CSIRO Food and Nutritional Sciences, P. O. Box 52, North Ryde, NSW 1670, Australia.
Arch Microbiol. 2012 Jun;194(6):513-23. doi: 10.1007/s00203-012-0788-z. Epub 2012 Jan 13.
Microbial diversity within formation water and oil from two compartments in Bokor oil reservoir from a Malaysian petroleum oil field was examined. A total of 1,056 16S rRNA gene clones were screened from each location by amplified ribosomal DNA restriction analysis. All samples were dominated by clones affiliated with Marinobacter, some novel Deferribacteraceae genera and various clones allied to the Methanococci. In addition, either Marinobacterium- or Pseudomonas-like operational taxonomic units were detected from either compartment. A systematic comparison with the existing pertinent studies was undertaken by analysing the microbial amplicons detected and the PCR primers used. The analyses demonstrated that bacterial communities were site specific, while Archaea co-occurred more frequently. Amplicons related to Marinobacter, Marinobacterium and Pseudomonas were detected in a number of the studies examined, suggesting they may be ubiquitous members in oil reservoirs. Further analysis of primers used in those studies suggested that most primer pairs had fairly broad but low matches across the bacterial and archaeal domains, while a minority had selective matches to certain taxa or low matches to all the microbial taxa tested. Thus, it indicated that primers may play an important role in determining which taxa would be detected.
对来自马来西亚油田 Bokor 油藏两个隔油层的地层水和油中的微生物多样性进行了研究。通过扩增核糖体 DNA 限制分析，从每个地点筛选了总共 1056 个 16S rRNA 基因克隆。所有样品均以 Marinobacter 属、一些新的 Deferribacteraceae 属和各种与 Methanococci 相关的克隆为主。此外，从任一个隔油层都检测到 Marinobacterium 或 Pseudomonas 样操作分类单元。通过分析检测到的微生物扩增子和使用的 PCR 引物，与现有相关研究进行了系统比较。分析表明，细菌群落具有特定的位置特异性，而古菌则更频繁地共存。在检查的许多研究中都检测到与 Marinobacter、Marinobacterium 和 Pseudomonas 相关的扩增子，这表明它们可能是油藏中普遍存在的成员。对这些研究中使用的引物进行进一步分析表明，大多数引物对在细菌和古菌领域具有相当广泛但匹配度较低的匹配，而少数引物对具有某些分类群的选择性匹配或对所有测试的微生物分类群的低匹配。因此，这表明引物可能在确定将检测到哪些分类群方面发挥重要作用。
