Department of Clinical Laboratory Medicine, Qilu Hospital of Shandong University, Jinan 250012, PR China.
Mol Med Rep. 2012 Apr;5(4):910-6. doi: 10.3892/mmr.2012.744. Epub 2012 Jan 9.
Immune inhibitory receptors play an important role in organ transplantation, autoimmune diseases and cancers. Immunoglobulin-like transcript (ILT)2 and ILT3 belong to the inhibitory receptors of the ILT family, which have been reported to regulate a broad range of cellular functions involved in the immune response. They contain immunoreceptor tyrosine-based inhibitory motifs (ITIMs), which are related to immune regulation. Although ILT receptors have been studied in dendritic cells (DCs), T cells, NK cells and other cell types, the expression and clinical significance of ILT2 and ILT3 in gastric cancer have yet to be elucidated. Here, the expression of ILT2 and ILT3 in gastric cancer cell lines and pathologic tissues, as well as their effects on the cytotoxicity of NK92MI against the gastric cancer cell lines MKNI with ILT2lowILT3low and HGC-27 with ILT2highILT3high were detected. The results suggest that ILT2 and ILT3 are expressed with diverse degrees in gastric cancer cells and tissues, and the expression of ILT2 is related with differentiation and size of tumors. Furthermore, the cytotoxic activity of NK92MI against the MKNI cell line was stronger than that against HGC-27. This study indicates that ILT2 and ILT3 play a key role in gastric cancer immune escape, and ILT2 may be a new target in the clinical diagnosis and treatment of gastric cancer.
免疫抑制受体在器官移植、自身免疫性疾病和癌症中发挥着重要作用。免疫球蛋白样转录物 (ILT)2 和 ILT3 属于 ILT 家族的抑制性受体，据报道它们可以调节广泛的与免疫反应相关的细胞功能。它们包含免疫受体酪氨酸抑制基序 (ITIM)，与免疫调节有关。尽管 ILT 受体已在树突状细胞 (DCs)、T 细胞、NK 细胞和其他细胞类型中进行了研究，但 ILT2 和 ILT3 在胃癌中的表达和临床意义尚未阐明。在这里，检测了 ILT2 和 ILT3 在胃癌细胞系和病理组织中的表达，以及它们对 NK92MI 对具有 ILT2lowILT3low 的胃癌细胞系 MKNI 和具有 ILT2highILT3high 的 HGC-27 的细胞毒性的影响。结果表明，ILT2 和 ILT3 在胃癌细胞和组织中以不同程度表达，ILT2 的表达与肿瘤的分化和大小有关。此外，NK92MI 对 MKNI 细胞系的细胞毒性活性强于对 HGC-27 的细胞毒性活性。本研究表明，ILT2 和 ILT3 在胃癌免疫逃逸中发挥着关键作用，ILT2 可能成为胃癌临床诊断和治疗的新靶点。
