Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287-5701, USA.
Proteomics. 2012 Feb;12(4-5):638-50. doi: 10.1002/pmic.201100381. Epub 2012 Jan 19.
Proteomics allows the assessment of cellular processes in an unprecedented scale by providing a comprehensive quantitative and qualitative overview of the protein content of a cell. Consequently, proteomics has been employed to investigate a multitude of bacterial processes ranging from the analysis of environmental communities, identification of virulence factors to the proteome-guided optimization of production strains. Proteomics has, in short, become an indispensable tool for the global analysis of bacterial physiology. Nonetheless, challenges exist, especially in the accurate prediction of phenotypic consequences based on any given proteome composition. In this review, we will give an overview of current highlights in the area of microbial proteomics, discuss some current challenges and present new developments that may help in overcoming them.
蛋白质组学通过全面定量和定性地了解细胞内的蛋白质含量，从而能够以前所未有的规模评估细胞的过程。因此，蛋白质组学已被用于研究多种细菌过程，从环境群落的分析、毒力因子的鉴定到生产菌株的蛋白质组导向优化。简而言之，蛋白质组学已成为细菌生理学全面分析不可或缺的工具。然而，仍存在一些挑战，特别是在基于给定蛋白质组组成准确预测表型后果方面。在这篇综述中，我们将概述微生物蛋白质组学领域的当前重点，讨论一些当前的挑战，并介绍可能有助于克服这些挑战的新进展。
