Otto Andreas, Becher Dörte, Schmidt Frank
Institute for Microbiology, Ernst Moritz Arndt University Greifswald, Germany.
Proteomics. 2014 Mar;14(4-5):547-65. doi: 10.1002/pmic.201300403.
Quantitative proteomics has become an indispensable analytical tool for microbial research. Modern microbial proteomics covers a wide range of topics in basic and applied research from in vitro characterization of single organisms to unravel the physiological implications of stress/starvation to description of the proteome content of a cell at a given time. With the techniques available, ranging from classical gel-based procedures to modern MS-based quantitative techniques, including metabolic and chemical labeling, as well as label-free techniques, quantitative proteomics is today highly successful in sophisticated settings of high complexity such as host-pathogen interactions, mixed microbial communities, and microbial metaproteomics. In this review, we will focus on the vast range of techniques practically applied in current research with an introduction of the workflows used for quantitative comparisons, a description of the advantages/disadvantages of the various methods, reference to hallmark publications and presentation of applications in current microbial research.
定量蛋白质组学已成为微生物研究中不可或缺的分析工具。现代微生物蛋白质组学涵盖了基础研究和应用研究中的广泛主题，从单个生物体的体外特性分析，以揭示应激/饥饿的生理影响，到描述给定时间细胞的蛋白质组含量。利用现有的技术，从经典的基于凝胶的方法到现代基于质谱的定量技术，包括代谢和化学标记以及无标记技术，定量蛋白质组学如今在宿主-病原体相互作用、混合微生物群落和微生物宏蛋白质组学等高复杂性的复杂环境中取得了巨大成功。在这篇综述中，我们将重点介绍当前研究中实际应用的广泛技术，介绍用于定量比较的工作流程，描述各种方法的优缺点，引用具有代表性的出版物，并展示当前微生物研究中的应用。
