气泡图揭示了噬菌体 φKZ 的内部结构。
Bubblegrams reveal the inner body of bacteriophage φKZ.
机构信息
Laboratory of Structural Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS), National Institutes of Health, Bethesda, MD 20892, USA.
出版信息
Science. 2012 Jan 13;335(6065):182. doi: 10.1126/science.1214120.
Abstract
Dense packing of macromolecules in cellular compartments and higher-order assemblies makes it difficult to pick out even quite large components in electron micrographs, despite nominally high resolution. Immunogold labeling and histochemical procedures offer ways to map certain components but are limited in their applicability. Here, we present a differential mapping procedure, based on the physical principle of protein's greater sensitivity to radiation damage compared with that of nucleic acid.
摘要
细胞区室和高级组装体中大分子的密集包装使得即使在名义上高分辨率的电子显微镜下也很难挑选出甚至相当大的成分。免疫金标记和组织化学程序提供了定位某些成分的方法，但在适用性方面受到限制。在这里，我们提出了一种基于蛋白质对辐射损伤的敏感性比核酸更高的物理原理的差异映射程序。
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