Department of Agriculture, Food and Resource Sciences, University of Maryland Eastern Shore, Princess Anne, Maryland, USA.
Appl Environ Microbiol. 2012 Mar;78(6):1675-81. doi: 10.1128/AEM.07304-11. Epub 2012 Jan 13.
Postharvest growth of Vibrio vulnificus in oysters can increase risk of human infection. Unfortunately, limited information is available regarding V. vulnificus growth and survival patterns over a wide range of storage temperatures in oysters harvested from different estuaries and in different oyster species. In this study, we developed a predictive model for V. vulnificus growth in Eastern oysters (Crassostrea virginica) harvested from Chesapeake Bay, MD, over a temperature range of 5 to 30°C and then validated the model against V. vulnificus growth rates (GRs) in Eastern and Asian oysters (Crassostrea ariakensis) harvested from Mobile Bay, AL, and Chesapeake Bay, VA, respectively. In the model development studies, V. vulnificus was slowly inactivated at 5 and 10°C with average GRs of -0.0045 and -0.0043 log most probable number (MPN)/h, respectively. Estimated average growth rates at 15, 20, 25, and 30°C were 0.022, 0.042, 0.087, and 0.093 log MPN/h, respectively. With respect to Eastern oysters, bias (B(f)) and accuracy (A(f)) factors for model-dependent and -independent data were 1.02 and 1.25 and 1.67 and 1.98, respectively. For Asian oysters, B(f) and A(f) were 0.29 and 3.40. Residual variations in growth rate about the fitted model were not explained by season, region, water temperature, or salinity at harvest. Growth rate estimates for Chesapeake Bay and Mobile Bay oysters stored at 25 and 30°C showed relatively high variability and were lower than Food and Agricultural Organization (FAO)/WHO V. vulnificus quantitative risk assessment model predictions. The model provides an improved tool for designing and implementing food safety plans that minimize the risk associated with V. vulnificus in oysters.
在牡蛎中，创伤弧菌的收获后生长会增加人类感染的风险。不幸的是，关于在不同河口收获的牡蛎和不同牡蛎物种中，创伤弧菌在广泛的储存温度范围内的生长和存活模式的信息有限。在这项研究中，我们开发了一个预测模型，用于预测来自马里兰州切萨皮克湾的东方牡蛎（Crassostrea virginica）在 5 至 30°C 的温度范围内的创伤弧菌生长情况，然后根据该模型对来自阿拉巴马州莫比尔湾和弗吉尼亚州切萨皮克湾的东方牡蛎和亚洲牡蛎（Crassostrea ariakensis）中创伤弧菌的生长速率（GR）进行了验证。在模型开发研究中，创伤弧菌在 5°C 和 10°C 下生长缓慢，平均 GR 分别为-0.0045 和-0.0043 log 最可能数（MPN）/h。在 15、20、25 和 30°C 下估计的平均生长速率分别为 0.022、0.042、0.087 和 0.093 log MPN/h。对于东方牡蛎，模型相关和独立数据的偏差（B(f)）和准确度（A(f)）因子分别为 1.02 和 1.25 以及 1.67 和 1.98。对于亚洲牡蛎，B(f)和 A(f)分别为 0.29 和 3.40。拟合模型的生长速率的剩余变化无法用收获时的季节、地区、水温或盐度来解释。在 25°C 和 30°C 下储存的切萨皮克湾和莫比尔湾牡蛎的生长率估计值显示出相对较高的变异性，并且低于粮农组织/世卫组织创伤弧菌定量风险评估模型的预测值。该模型为设计和实施食品安全计划提供了一种改进的工具，可最大程度地降低牡蛎中创伤弧菌相关风险。
