Sahu Rohit, Divakar Goli, Divakar Kalyani
Acharya & B.M. Reddy College of Pharmacy, Bangalore (India).
J Adv Pharm Technol Res. 2010 Jan;1(1):22-9.
Gmelina arborea Roxb (family Verbenaceae) commonly known as 'Gambhari' tree, the various parts of the plants are widely used in diarrhoea, anti-pyretic, thirst, anemia, leprosy, ulcers, consumption, strangury, vaginal discharges. We tested the genotoxic potential of G. arborea in bone marrow cells obtained from Swiss albino mice using micronuclei formation as the toxicological endpoints. Aqueous extract of G. arborea (AEGA) was tested at the dose of 286 & 667 mg/kg body weight (b. w.). Cyclophosphamide (CPZ) 25 mg/kg b. w. was used as positive control in micronucleus test. The AEGA significantly increased the % micronucleated polychrometics at doses of 286mg/kg and 667mg/kg, after 24, 48 72h time interval. And also decreased the PCE/NCE ratio after 24, 48 and 72 h as compared to solvent control group. In this study, we investigated the effect of G. arborea on mammalian bone marrow cells using micronuclei formation to assess the genotoxicity of the herb.
柚木（马鞭草科）通常被称为“甘巴里”树，其植株的各个部分被广泛用于治疗腹泻、退热、解渴、贫血、麻风病、溃疡、痨病、尿痛、白带。我们以微核形成作为毒理学终点，测试了柚木对瑞士白化小鼠骨髓细胞的遗传毒性潜力。以286和667毫克/千克体重的剂量测试了柚木水提取物（AEGA）。在微核试验中，25毫克/千克体重的环磷酰胺（CPZ）用作阳性对照。在24、48、72小时的时间间隔后，286毫克/千克和667毫克/千克剂量的AEGA显著增加了多染红细胞微核率。与溶剂对照组相比，在24、48和72小时后，AEGA也降低了嗜多染红细胞/嗜中性红细胞比率。在本研究中，我们利用微核形成来评估该草药的遗传毒性，研究了柚木对哺乳动物骨髓细胞的影响。
