使用Neurolucida进行神经元成像——一种基于个人电脑的图像合成显微镜系统。
Neuron imaging with Neurolucida--a PC-based system for image combining microscopy.
作者信息
Glaser J R, Glaser E M
机构信息
University of Vermont, Department of Anatomy and Neurobiology, College of Medicine, Burlington 05477.
出版信息
Comput Med Imaging Graph. 1990 Sep-Oct;14(5):307-17. doi: 10.1016/0895-6111(90)90105-k.
Neurolucida is a new software package for performing 3-D neuron mapping and tracing to 0.5 micron precision through the oculars of a microscope. It incorporates computer controlled stage movement in three dimensions. Sections are limited in size only by the stage dimensions. An on-line, direct view image editor is provided as are dynamic image rotations in color and morphometric analyses. Neurolucida is written in 'C' and runs on PC ATs, XTs, and PS/2s using DOS and VGA, EGA, or NTSC graphics. Neurolucida has a video mode compatible with frame grabbers, thereby permitting its application to video microscopy.
NeuroLucida是一款新的软件包，用于通过显微镜目镜进行三维神经元映射和追踪，精度可达0.5微米。它集成了计算机控制的三维载物台移动。切片大小仅受载物台尺寸限制。提供了一个在线直接视图图像编辑器，以及彩色动态图像旋转和形态计量分析功能。NeuroLucida用“C”语言编写，可在使用DOS和VGA、EGA或NTSC图形的PC AT、XT和PS/2上运行。NeuroLucida具有与图像采集卡兼容的视频模式，因此可应用于视频显微镜。
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