[高良姜的高效液相色谱指纹图谱研究]
[Study on HPLC fingerprint of Alpinia officinarum].
作者信息
Deng Yi-Feng, Feng Li-Na, Luo Hui
机构信息
Guangdong Medical College, Key laboratory of Natural Drugs Research and Development of Guangdong, Zhanjiang 524023, China.
出版信息
Zhong Yao Cai. 2011 Sep;34(9):1351-5.
OBJECTIVE
To establish the chromatography fingerprint of Alpinia officinarum by HPLC.
METHODS
An optimum HPLC conditions which were obtained under the assessment of LC-MS were as follows: Shim-pack VP-ODS column (2.0 mm x 250 mm, 5 microm), 0.1% HAc aqueous solution as phase A, 15% Acetonitrile: 40% Methanol: 45% Tetrafuran as phase B, the flow rate was 0.20 mL/min, column temperature was 35 degrees C and UV detector was set at 280 nm.
RESULTS
The HPLC fingerprint of Alpinia officinarum was established, the consensus 10 peaks and their relative retention times along with the ranges of relative area were determined.
CONCLUSION
The method is reliable and stable and can be used for the quality control and identification of Alpinia officinarum.
目的
采用高效液相色谱法建立高良姜的色谱指纹图谱。
方法
在液质联用仪评估下获得的最佳高效液相色谱条件如下：Shim-pack VP-ODS柱（2.0 mm×250 mm，5微米），以0.1%醋酸水溶液为A相，15%乙腈：40%甲醇：45%四氢呋喃为B相，流速为0.20 mL/min，柱温为35℃，紫外检测器设定在280 nm。
结果
建立了高良姜的高效液相色谱指纹图谱，确定了10个共有峰及其相对保留时间以及相对面积范围。
结论
该方法可靠、稳定，可用于高良姜的质量控制和鉴别。
Zotero 插件