[新疆维吾尔药腺毛黑种草籽的高效液相色谱指纹图谱]
[HPLC fingerprint spectrum of xinjiang uyghur medicine Nigella glandulifera seed].
作者信息
Tan Xiu-Fang, Li Xiao-Jin, Wang Qiang, Shi Ming-Hui, Yang Jie, Cao Yuan
机构信息
College of Pharmacy, Xinjiang Medical University, Urmqi 830054, China.
出版信息
Zhong Yao Cai. 2011 Dec;34(12):1857-60.
OBJECTIVE
To establish the HPLC fingerprint spectrum of Nigella glandulifera seed identification.
METHODS
The chromatographic system consisted of a Shim-pack ODS C18 column (150 mm x 4.6 mm, 5 microm) and a mobile phase of acetonitrile-0.1% formic acid in condition of gradient elution,The detection wavelength was 280 nm. The column temperature was 30 degrees C.
RESULTS
Detected the HPLC fingerprint spectrum of 10 batches samples Nigella glandulifera seed from Xinjiang; Calibrated 18 characteristic peaks. The result was in accordance with the technical requirement of fingerprint spectrum. The similarity calculation software showed that the similarity was higher than 0. 95.
CONCLUSION
The HPLC fingerprint spectrum of Nigella glandulifera seed can be used as an identification method. It may provide the basis for quality control of Nigella glandulifera seed.
目的
建立腺毛黑种草籽的高效液相色谱指纹图谱用于鉴别。
方法
色谱系统由Shim-pack ODS C18柱（150 mm×4.6 mm，5μm）和乙腈-0.1%甲酸组成的流动相，采用梯度洗脱条件，检测波长为280 nm，柱温为30℃。
结果
检测了10批新疆腺毛黑种草籽的高效液相色谱指纹图谱；标定了18个特征峰。结果符合指纹图谱的技术要求。相似度计算软件显示相似度高于0.95。
结论
腺毛黑种草籽的高效液相色谱指纹图谱可作为一种鉴别方法。可为腺毛黑种草籽的质量控制提供依据。
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