Feng Juan, Fan Yuqin, Ayiheng Qukuerhan, Zhang Hua, Yong Jun, Hu Bin
Department of Otorhinolaryngology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uyghur 830054, P.R. China.
Oncol Lett. 2017 Jul;14(1):480-486. doi: 10.3892/ol.2017.6155. Epub 2017 May 11.
A majority of studies have indicated that microRNA-125b (miR-125b) is aberrantly expressed in various types of cancer. However, there are no studies on the expression and function of miR-125b in human laryngeal squamous cell carcinoma (LSCC). In the present study, miR-125b expression in LSCC sample tissues, corresponding adjacent non-neoplastic tissues, LSCC cell lines and a normal human keratinocyte cell line was measured using the reverse transcription-quantitative polymerase chain reaction. Following transfection with miR-125b mimics, the Cell Counting Kit-8, cell migration, cell invasion, western blotting and dual-luciferase reporter assays were performed on LSCC cell lines. According to the results, miR-125b was observed to be significantly downregulated in LSCC, and its expression was significantly associated with clinical stage and alcohol history. miR-125b was also observed to decrease cell growth, migration and invasion in LSCC cells by directly targeting signal transducer and activator of transcription 3. The results of the present study suggested that miR-125b may be a potential treatment target of LSCC in the future.
大多数研究表明，微小RNA-125b（miR-125b）在多种类型的癌症中表达异常。然而，关于miR-125b在人喉鳞状细胞癌（LSCC）中的表达及功能尚无研究。在本研究中，采用逆转录-定量聚合酶链反应检测LSCC样本组织、相应的癌旁非肿瘤组织、LSCC细胞系及正常人角质形成细胞系中miR-125b的表达。用miR-125b模拟物转染后，对LSCC细胞系进行细胞计数试剂盒-8、细胞迁移、细胞侵袭、蛋白质印迹和双荧光素酶报告基因检测。结果显示，miR-125b在LSCC中显著下调，其表达与临床分期和饮酒史显著相关。还观察到miR-125b通过直接靶向信号转导和转录激活因子3降低LSCC细胞的生长、迁移和侵袭。本研究结果表明，miR-125b未来可能成为LSCC的潜在治疗靶点。
