Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, Universidade Federal do Rio Grande do Sul, 91501-970 Porto Alegre, Brazil.
Nat Prod Res. 2012;26(23):2259-65. doi: 10.1080/14786419.2011.652958. Epub 2012 Jan 19.
Cerein 8A is an antimicrobial peptide with potential application against food spoilage and pathogenic bacteria. The partitioning of cerein 8A was investigated in two liquid-liquid extraction systems that are considered promising for bioseparation and purification purposes. Aqueous two-phase systems (ATPSs) were prepared with polyethylene glycol (PEG) and inorganic salts, and the addition of NaCl was investigated in this system. The best results concerning partition coefficients (K (b)) were obtained with PEG + ammonium sulphate, and K (b) value significantly increases when NaCl was added. Cerein 8A was effectively extracted into the micelle-rich phase in a 4% Triton X-114 medium. Recovery yield was higher for ATPS compared to micellar systems. Cerein 8A can be isolated from a crude suspension containing the bioactive molecule by ATPSs. Successful implementation of peptide partitioning represents an important step towards developing a low-cost effective separation method for cerein 8A.
Cerein 8A 是一种具有潜在应用价值的抗菌肽，可以对抗食物腐败和致病菌。本研究考察了两种液-液萃取系统中 Cerein 8A 的分配情况，这两种系统有望用于生物分离和纯化目的。采用聚乙二醇（PEG）和无机盐制备双水相体系（ATPS），并在此系统中考察了添加 NaCl 的效果。PEG+硫酸铵体系获得的分配系数（K（b））最佳，当添加 NaCl 时，K（b）值显著增加。Cerein 8A 可以有效地萃取到 4% Triton X-114 介质中的胶束富相。与胶束体系相比，ATPS 的回收率更高。Cerein 8A 可以从含有生物活性分子的粗悬浮液中通过 ATPS 进行分离。肽分配的成功实施代表着朝着开发低成本有效分离 Cerein 8A 的方法迈出了重要一步。
