Kim Jong-Hui, Lee Eun-Seon, Kim Bu-Min, Ham Jun-Sang, Oh Mi-Hwa
Animal Products Utilization Division, National Institute of Animal Science, RDA, Wanju 55365, Republic of Korea.
Foods. 2023 Dec 19;13(1):10. doi: 10.3390/foods13010010.
Bacteriocins may be used as natural preservatives and antibiotic substitutes in various foods. However, the multistep purification process of bacteriocins results in high production costs, which is an obstacle to their commercial use and consumer accessibility. In this study, a bacteriocin-like inhibitory substance (BLIS) from spp. isolated from Korean fermented foods was partially purified using the aqueous two-phase system (ATPS). The maximum activity of the BLIS was achieved for ATPS composed of PEG 1000 (15% [/])/ammonium sulfate (20% [/])/sodium chloride (2% [/]), which caused BLIS activity to increase by 3 times with a 99% recovery rate. In particular, Y138-6 BLIS exhibited broad antibacterial activity, high resistance to acid-base stress, and excellent thermal stability. This antibacterial substance inhibited the growth of aerobic bacteria and fungi on the walls of cheese and ripening rooms. These antibacterial properties have been shown to increase food safety and have the potential for use as biopreservatives. Moreover, considering that the execution of the ATPS requires only salts and PEG, it is a simple, environmentally friendly, and cost-effective process and may have industrial applications in the recovery of BLIS from fermentation broth.
细菌素可作为各种食品中的天然防腐剂和抗生素替代品。然而，细菌素的多步纯化过程导致生产成本高昂，这是其商业应用和消费者获取的障碍。在本研究中，使用双水相系统（ATPS）对从韩国发酵食品中分离出的 spp. 产生的一种类细菌素抑制物质（BLIS）进行了部分纯化。由聚乙二醇1000（15%[/]）/硫酸铵（20%[/]）/氯化钠（2%[/]）组成的ATPS实现了BLIS的最大活性，使BLIS活性提高了3倍，回收率达99%。特别是，Y138 - 6 BLIS表现出广泛的抗菌活性、对酸碱胁迫的高耐受性以及出色的热稳定性。这种抗菌物质抑制了奶酪壁和成熟室中需氧菌和真菌的生长。这些抗菌特性已被证明可提高食品安全，并具有用作生物防腐剂的潜力。此外，考虑到ATPS的实施仅需盐和聚乙二醇，它是一个简单、环保且经济高效的过程，可能在从发酵液中回收BLIS方面具有工业应用价值。
