口腔病变与健康口腔黏膜中人乳头瘤病毒亚型的比较。
Human papillomavirus subtypes in oral lesions compared to healthy oral mucosa.
机构信息
Department of Oral Biology, University of Oslo, 0316 Oslo, Norway.
出版信息
J Clin Virol. 2012 Apr;53(4):364-6. doi: 10.1016/j.jcv.2011.12.023. Epub 2012 Jan 18.
BACKGROUND
Human papillomaviruses (HPV) are involved in the etiology of cervix cancer, but it is still unclear whether they play a role in related oral lesions.
OBJECTIVES
The presence of HPV in oral leukoplakia biopsies (n=50) and oral squamous carcinoma biopsies (n=50) was compared to normal oral mucosa swabs (n=50) for the purpose of indicating a possible etiological role for the virus.
STUDY DESIGN
DNA was extracted from tissue biopsies and from mucosa swabs of control samples. Nested PCR was performed with primers targeting conserved sequences within the capsid gene L1. PCR products were sequenced to identify the HPV genotype.
RESULT
The results reveal a profile of low-risk HPV genotypes in oral leukoplakia similar to that in healthy controls, while HPV was less frequently observed in oral squamous carcinoma.
CONCLUSIONS
HPV does not seem to represent an important causal factor for the development of oral leukoplakia or oral squamous carcinoma.
背景
人乳头瘤病毒（HPV）与宫颈癌的病因有关，但目前尚不清楚它们是否在相关的口腔病变中发挥作用。
目的
比较口腔白斑病活检组织（n=50）和口腔鳞状细胞癌活检组织（n=50）与正常口腔黏膜拭子（n=50）中 HPV 的存在，以提示病毒可能具有的病因学作用。
研究设计
从组织活检和对照样本的黏膜拭子中提取 DNA。用针对衣壳基因 L1 内保守序列的引物进行巢式 PCR。对 PCR 产物进行测序以鉴定 HPV 基因型。
结果
结果显示，口腔白斑病中 HPV 基因型呈低危型谱，与健康对照组相似，而 HPV 在口腔鳞状细胞癌中则较少见。
结论
HPV 似乎不是口腔白斑病或口腔鳞状细胞癌发展的重要致病因素。
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