College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China.
Department of Food Science and Technology, National University of Singapore, 117543, Singapore.
Food Chem. 2023 Sep 30;421:136148. doi: 10.1016/j.foodchem.2023.136148. Epub 2023 Apr 17.
The polyphenols extracted from 20 blue honeysuckle cultivars were comprehensively characterized and quantified by HPLC-DAD and HPLC-ESI-QTOF-MS analyses and evaluated for antioxidant capacity (ABTS, DPPH, FRAP) and α-amylase inhibitory activity. The 17 anthocyanins and 59 non-anthocyanin phenolics were characterized. Among them, cyanidin-3-glucoside, quercetin-3-galactoside, myricetin-3-galactoside, and 3-caffeoylquinic acid were the major polyphenols. These polyphenols not only contributed to the antioxidant capacity, but were also good α-amylase inhibitors. 'Lanjingling' showed the strongest antioxidant capacity evaluated by FRAP, while 'CBS-2' and '14-13-1' showed the strongest antioxidant capacity evaluated by ABTS and DPPH. All the twenty cultivars showed α-amylase inhibitory activity, and the IC values ranged from 0.12 ± 0.01 to 0.69 ± 0.02 mg/mL. 'Lanjingling' showed the most potent α-amylase inhibitory activity. Additionally, principal component analysis indicated that Lonicera. caerulea subsp. emkuyedao bred in Japan differed markedly in phenolics and bioactivity compared to the other four subspecies bred in China and Russia.
采用高效液相色谱-二极管阵列检测（HPLC-DAD）和高效液相色谱-电喷雾串联四极杆飞行时间质谱联用（HPLC-ESI-QTOF-MS）分析对 20 个蓝靛果品种的多酚进行了综合特征描述和定量分析，并评估了其抗氧化能力（ABTS、DPPH、FRAP）和α-淀粉酶抑制活性。鉴定出 17 种花色苷和 59 种非花色苷酚类物质。其中，矢车菊素-3-葡萄糖苷、槲皮素-3-半乳糖苷、杨梅素-3-半乳糖苷和 3-咖啡酰奎宁酸是主要的多酚类物质。这些多酚类物质不仅有助于抗氧化能力，而且还是良好的α-淀粉酶抑制剂。'蓝靛果 1 号'的 FRAP 评价抗氧化能力最强，而'CBS-2'和'14-13-1'的 ABTS 和 DPPH 评价抗氧化能力最强。所有 20 个品种均表现出α-淀粉酶抑制活性，IC 值范围为 0.12±0.01 至 0.69±0.02 mg/mL。'蓝靛果 1 号'表现出最强的α-淀粉酶抑制活性。此外，主成分分析表明，在日本培育的蓝靛果亚种 emkuyedao 在酚类物质和生物活性方面与在中国和俄罗斯培育的其他 4 个亚种明显不同。
