Institute of Animal Nutrition, Vetsuisse-Faculty University of Zurich, Zurich, Switzerland.
J Anim Physiol Anim Nutr (Berl). 2012 Oct;96(5):860-9. doi: 10.1111/j.1439-0396.2011.01269.x. Epub 2012 Jan 20.
The aim of the present study was to compare carbohydrate degradation of forages which store carbohydrates either predominantly as fructan or starch, in horses' hindgut. The effects of an abrupt change from hay-based feeding to green fodder-based feeding on the caecal flora were tested with the in vitro hindgut simulation technique 'Caesitec'. Six trials with different forages (English ryegrass, tall fescue, grass mixture-horses, grass mixture-cows, lucerne, white clover) were conducted. During a 4-day stabilisation period, samples were taken once a day before loading the fermenters with hay. After diet-change to forage-based feeding, samples were taken four times a day. Ammonia and pH-value were measured before and 1, 2 and 6 h after loading the 'Caesitec'. Gas formation was measured daily. Bacterial numbers, lactate and short chain fatty acids were detected at four time-points of each trial. The grass mixtures contained the highest amounts of fructan. The pH-values were in the physiological range from pH 6 up to 7 (6.58-6.83) by feeding all forages. Gas formation, anaerobic and aerobic bacterial numbers increased after diet change from hay to any forage. The maximum amount of fructan (3.75 g/kg) in swiss pasture did not cause a permanent pathological change in the hindgut-flora.
本研究旨在比较在马后肠中以果聚糖或淀粉为主储存碳水化合物的饲草的碳水化合物降解情况。采用体外后肠模拟技术“Caesitec”，测试了从干草喂养突然转变为绿色饲料喂养对盲肠菌群的影响。进行了六次不同饲草（英国黑麦草、高羊茅、马用混合草、奶牛用混合草、紫花苜蓿、白三叶草）的试验。在 4 天的稳定期内，每天在向发酵罐中装入干草之前取样一次。在改为饲草喂养后，每天取样四次。在装入“Caesitec”前和装入后 1、2 和 6 小时测量氨和 pH 值。每天测量气体形成量。在每个试验的四个时间点检测细菌数量、乳酸和短链脂肪酸。混合草含有最高量的果聚糖。通过喂养所有饲草，pH 值在生理范围内，从 6 上升到 7（6.58-6.83）。从干草改为任何饲草后，气体形成、厌氧和需氧细菌数量增加。瑞士牧场中最高含量的 3.75 克/千克果聚糖并没有在后肠菌群中引起永久性病理变化。
