Laboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Respir Physiol Neurobiol. 2012 Jun 15;182(1):26-36. doi: 10.1016/j.resp.2012.01.002. Epub 2012 Jan 13.
We hypothesized that bone marrow-derived mononuclear cell (BMDMC) therapy protects the lung and consequently the heart in experimental elastase-induced emphysema. Twenty-four female C57BL/6 mice were intratracheally instilled with saline (C group) or porcine pancreatic elastase (E group) once a week during 4 weeks. C and E groups were randomized into subgroups receiving saline (SAL) or male BMDMCs (2 × 10(6), CELL) intravenously 3h after the first saline or elastase instillation. Compared to E-SAL group, E-CELL mice showed, at 5 weeks: lower mean linear intercept, neutrophil infiltration, elastolysis, collagen fiber deposition in alveolar septa and pulmonary vessel wall, lung cell apoptosis, right ventricle wall thickness and area, higher endothelial growth factor and insulin-like growth factor mRNA expressions in lung tissue, and reduced platelet-derived growth factor, transforming growth factor-β, and caspase-3 expressions. In conclusion, BMDMC therapy was effective at modulating the inflammatory and remodeling processes in the present model of elastase-induced emphysema.
我们假设骨髓来源的单核细胞（BMDMC）治疗可保护肺，进而保护实验性弹性蛋白酶诱导的肺气肿中的心脏。24 只雌性 C57BL/6 小鼠每周通过气管内滴注生理盐水（C 组）或猪胰腺弹性蛋白酶（E 组）一次，持续 4 周。C 组和 E 组随机分为亚组，在第一次生理盐水或弹性蛋白酶滴注后 3 小时分别静脉内给予生理盐水（SAL）或雄性 BMDMC（2×10(6)，CELL）。与 E-SAL 组相比，E-CELL 组在第 5 周时表现出：平均线性截距降低、中性粒细胞浸润减少、弹性酶降解减少、肺泡隔和肺血管壁胶原纤维沉积减少、肺细胞凋亡减少、右心室壁厚度和面积减少、肺组织中内皮生长因子和胰岛素样生长因子 mRNA 表达增加，血小板衍生生长因子、转化生长因子-β和半胱氨酸天冬氨酸蛋白酶-3 表达减少。总之，BMDMC 治疗在本弹性蛋白酶诱导的肺气肿模型中有效调节了炎症和重塑过程。
