Laboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Respir Physiol Neurobiol. 2013 Jun 15;187(2):190-8. doi: 10.1016/j.resp.2013.03.014. Epub 2013 Mar 30.
We compared the effects of bone marrow-derived mononuclear cells (BMMCs) and mesenchymal stromal cells (MSCs) on airway inflammation and remodeling and lung mechanics in experimental allergic asthma. C57BL/6 mice were sensitized and challenged with ovalbumin (OVA group). A control group received saline using the same protocol. Twenty-four hours after the last challenge, groups were further randomized into subgroups to receive saline, BMMCs (2×10(6)) or MSCs (1×10(5)) intratracheally. BMMC and MSC administration decreased cell infiltration, bronchoconstriction index, alveolar collapse, collagen fiber content in the alveolar septa, and interleukin (IL)-4, IL-13, transforming growth factor (TGF)-β and vascular endothelial growth factor (VEGF) levels compared to OVA-SAL. Lung function, alveolar collapse, collagen fiber deposition in alveolar septa, and levels of TGF-β and VEGF improved more after BMMC than MSC therapy. In conclusion, intratracheal BMMC and MSC administration effectively modulated inflammation and fibrogenesis in an experimental model of asthma, but BMMCs was associated with greater benefit in terms of reducing levels of fibrogenesis-related growth factors.
我们比较了骨髓来源的单核细胞（BMMCs）和间充质基质细胞（MSCs）对实验性变应性哮喘中气道炎症和重塑及肺力学的影响。C57BL/6 小鼠用卵清蛋白（OVA）致敏和激发。对照组采用相同方案给予生理盐水。末次激发 24 小时后，各组进一步随机分为亚组，给予生理盐水、BMMC（2×10（6））或 MSC（1×10（5））气管内滴注。与 OVA-SAL 相比，BMMC 和 MSC 给药可减少细胞浸润、支气管收缩指数、肺泡塌陷、肺泡隔胶原纤维含量以及白细胞介素（IL）-4、IL-13、转化生长因子（TGF）-β 和血管内皮生长因子（VEGF）水平。与 MSC 治疗相比，BMMC 治疗后肺功能、肺泡塌陷、肺泡隔胶原纤维沉积以及 TGF-β 和 VEGF 水平改善更明显。总之，气管内 BMMC 和 MSC 给药可有效调节哮喘模型中的炎症和纤维发生，但 BMMC 与降低纤维化相关生长因子水平相关的获益更大。
