School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
Nano Lett. 2013 Jan 9;13(1):1-8. doi: 10.1021/nl203780d. Epub 2012 Feb 2.
The ability to monitor intracellular events in real time is paramount to advancing fundamental biological and clinical science. We present the first demonstration of a direct interface of vertically aligned single-walled carbon nanotubes (VASWCNTs) with eukaryotic cells, RAW 264.7 mouse macrophage cell line. The cells were cultured on indium tin oxide with VASWCNTs. VASWCNTs entered the cells naturally without application of any external force and were shown to sense the intracellular presence of a redox active moiety, methylene blue. The technology developed provides an alluring platform to enable electrochemical study of an intracellular environment.
实时监测细胞内事件对于推进基础生物学和临床医学至关重要。我们首次展示了垂直排列的单壁碳纳米管（VASWCNTs）与真核细胞（RAW 264.7 小鼠巨噬细胞系）的直接接口。将细胞培养在具有 VASWCNTs 的氧化铟锡上。VASWCNTs 无需施加任何外力即可自然进入细胞，并被证明可以检测到细胞内存在氧化还原活性部分，亚甲蓝。所开发的技术为实现细胞内环境的电化学研究提供了一个极具吸引力的平台。
