Department of Industrial Biological Chemistry, Gembloux Agro-Bio Tech, University of Liège, Passage des Déportés 2, B-5030, Gembloux, Belgium.
Appl Biochem Biotechnol. 2012 Mar;166(6):1423-32. doi: 10.1007/s12010-011-9535-1. Epub 2012 Jan 22.
The effect of two types of cellulose, microcrystalline cellulose and paper pulp, on enzymatic hydrolysis for cellobiose production was investigated. The particle size, the relative crystallinity index and the water retention value were determined for both celluloses. A previously studied multistage hydrolysis process that proved to enhance the cellobiose production was studied with both types of celluloses. The cellobiose yield exhibited a significant improvement (120% for the microcrystalline cellulose and 75% for the paper pulp) with the multistage hydrolysis process compared to continuous hydrolysis. The conversion of cellulose to cellobiose was greater for the microcrystalline cellulose than for the paper pulp. Even with high crystallinity, microcrystalline cellulose achieved the highest cellobiose yield probably due to its highest specific surface area accessible to enzymes and quantity of adsorbed protein.
研究了两种纤维素（微晶纤维素和纸浆）对生产纤维二糖的酶水解的影响。测定了两种纤维素的粒径、相对结晶度指数和保水值。研究了一种已被证明能提高纤维二糖产量的多阶段水解工艺，该工艺分别用于这两种纤维素。与连续水解相比，多阶段水解过程使纤维二糖的得率显著提高（微晶纤维素提高了 120%，纸浆提高了 75%）。微晶纤维素转化为纤维二糖的转化率高于纸浆。即使结晶度很高，微晶纤维素也能获得最高的纤维二糖产率，这可能是由于其对酶具有最高的比表面积和吸附蛋白量。
