Lanzou Institute of Biological Products, Lanzhou 730070, China.
Virol Sin. 2012 Feb;27(1):10-8. doi: 10.1007/s12250-012-3206-4. Epub 2012 Jan 22.
Enterovirus 71 (EV71) is a member of the Entero-virus genus of the Picornaviridae family and is the major cause of Hand, foot, and mouth disease (HFMD) in children. Different strains from Gansu were cloned and the P1 protein was sequenced and analysed. Results indicate that there are three kinds of EV71 infections prevalent in Gansu. The VP1 protein from one of these strains, 55F, was expressed. The recombinant protein was expressed with high level and reacted specifically with the EV71 patient antibody, the recombinant protein was also applied to raise antiserum in rabbits and after the fourth injection a high titer of antiserum was detected by ELISA assay. These data are useful for further clarification of prevalent EV71 strains in the north of China at the molecular level and provide a basis for EV71 diagnosis.
肠道病毒 71 型（EV71）属于小 RNA 病毒科肠道病毒属，是引起儿童手足口病（HFMD）的主要病原体。本研究对来自甘肃的不同分离株进行了克隆，并对 P1 蛋白进行了测序和分析。结果表明，甘肃流行着三种 EV71 感染。对其中一株，55F 株的 VP1 蛋白进行了表达。重组蛋白表达水平较高，且能与 EV71 患者抗体特异性反应，该重组蛋白还被用于兔抗体制备，经 4 次免疫后，ELISA 检测到了较高滴度的血清抗体。这些数据有助于进一步阐明中国北方流行的 EV71 株在分子水平上的情况，为 EV71 的诊断提供了依据。
