Department of Cytobiochemistry, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
Pathol Oncol Res. 2012 Jul;18(3):721-8. doi: 10.1007/s12253-012-9500-5. Epub 2012 Jan 21.
Cancer cells have accelerated metabolism and high glucose requirements. The up-regulation of specific glucose transporters may represent a key mechanism by which malignant cells may achieve increased glucose uptake to support the high rate of glycolysis. In present study we analyzed the mRNA and protein expression of GLUT1 and GLUT3 glucose transporters by quantitative real-time polymerase chain reaction (Q-PCR) and Western blotting technique in 76 cases of endometrial carcinoma and 70 cases of breast carcinoma. SLC2A1 and SLCA2A3 mRNAs expression was found, respectively in 100% and 97.4% samples of endometrial cancers and only in 50% and 40% samples of breast cancers. In endometrial cancers GLUT1 and GLUT3 protein expression was identified in 67.1% and 30.3% of cases. Analogously, in breast cancers in 48.7% and 21% of samples, respectively. The results showed that both endometrial and breast poorly differentiated tumors (grade 2 and 3) had significantly higher GLUT1 and GLUT3 expression than well-differentiated tumors (grade 1). Statistically significant association was found between SLCA2A3 mRNA expression and estrogen and progesterone receptors status in breast cancers. GLUT1 has been reported to be involved in the uptake of glucose by endometrial and breast carcinoma cells earlier and the present study determined that GLUT3 expression is also involved. GLUT1 and GLUT3 seem to be important markers in endometrial and breast tumors differentiation.
癌细胞具有加速的新陈代谢和高葡萄糖需求。特定葡萄糖转运体的上调可能代表恶性细胞可能增加葡萄糖摄取以支持高糖酵解率的关键机制。在本研究中，我们通过定量实时聚合酶链反应（Q-PCR）和 Western 印迹技术分析了 76 例子宫内膜癌和 70 例乳腺癌中 GLUT1 和 GLUT3 葡萄糖转运体的 mRNA 和蛋白表达。在 100％的子宫内膜癌和仅在 50％的乳腺癌样本中分别发现了 SLC2A1 和 SLCA2A3 mRNA 的表达。在子宫内膜癌中，鉴定出 GLUT1 和 GLUT3 蛋白表达分别为 67.1％和 30.3％。类似地，在乳腺癌中，分别为 48.7％和 21％。结果表明，无论是子宫内膜癌还是乳腺癌低分化肿瘤（等级 2 和 3），GLUT1 和 GLUT3 的表达均明显高于高分化肿瘤（等级 1）。在乳腺癌中发现 SLCA2A3 mRNA 表达与雌激素和孕激素受体状态之间存在统计学显著关联。先前有报道称 GLUT1 参与了子宫内膜癌和乳腺癌细胞对葡萄糖的摄取，本研究确定 GLUT3 表达也参与其中。GLUT1 和 GLUT3 似乎是子宫内膜和乳腺癌肿瘤分化的重要标志物。
