Goldman Noah A, Katz Ellen B, Glenn Alan S, Weldon Richard H, Jones Joan G, Lynch Uticia, Fezzari Melissa J, Runowicz Carolyn D, Goldberg Gary L, Charron Maureen J
Division of Gynecologic Oncology, Department of Obstetrics & Gynecology, Albert Einstein College of Medicine and Montefiore Medical Center, Albert Einstein Comprehensive Cancer Center, Bronx, NY 10461, USA.
Mod Pathol. 2006 Nov;19(11):1429-36. doi: 10.1038/modpathol.3800656. Epub 2006 Aug 4.
Glucose is provided to cells by a family of glucose transport facilitators known as GLUTs. These transporters are expressed in a tissue specific manner and are overexpressed in many primary tumors of these tissues. Regulation of glucose transport facilitator expression has been demonstrated in endometrial tissue and endometrial adenocarcinoma. The following experiments were conducted to quantify and localize the expression of GLUT1 and GLUT8 in benign endometrium and compare this expression to endometrial cancer. Endometrial tissue samples were obtained from random hysterectomy specimens of patients with benign indications for surgery and endometrial cancer. Immunoblot and immunolocatization studies were performed using GLUT1 and GLUT8 specific antisera. Endometrial samples from 65 women who had undergone hysterectomy were examined (n=38 benign, n=27 malignant). A 44 and a 35.4 kDa immunoreacive species was demonstrated in endometrium and endometrial cancer for GLUT1 and GLUT8, respectively. Upregulation of GLUT1 expression was demonstrated with increasing grade of tumors (P<0.002). GLUT8 expression was increased in all tumor subtypes compared to atrophic endometrium (P<0.001). Apical localization by GLUT1 and GLUT8 was demonstrated in endometrial glands. GLUT1 and GLUT8 demonstrated diffuse intracellular localization in the cancer subtypes. GLUT1 and GLUT8 are expressed in both human endometrium and endometrial cancer. There appears to be a step-wise progression in GLUT1 and GLUT8 expression as tumor histopathology worsens. GLUT1 and GLUT8 may be important markers in tumor differentiation, as well as providing energy to rapidly dividing tumor cells.
葡萄糖通过一类称为葡萄糖转运体（GLUTs）的葡萄糖转运促进因子家族提供给细胞。这些转运体以组织特异性方式表达，并且在这些组织的许多原发性肿瘤中过度表达。已在子宫内膜组织和子宫内膜腺癌中证实了葡萄糖转运促进因子表达的调节。进行以下实验以定量和定位GLUT1和GLUT8在良性子宫内膜中的表达，并将这种表达与子宫内膜癌进行比较。从有良性手术指征的患者和子宫内膜癌患者的随机子宫切除标本中获取子宫内膜组织样本。使用GLUT1和GLUT8特异性抗血清进行免疫印迹和免疫定位研究。检查了65例接受子宫切除术的女性的子宫内膜样本（n = 38例良性，n = 27例恶性）。在子宫内膜和子宫内膜癌中分别证实了GLUT1和GLUT8的44 kDa和35.4 kDa免疫反应性条带。随着肿瘤分级增加，GLUT1表达上调（P<0.002）。与萎缩性子宫内膜相比，所有肿瘤亚型中GLUT8表达均增加（P<0.001）。在子宫内膜腺中证实了GLUT1和GLUT8的顶端定位。在癌症亚型中，GLUT1和GLUT8表现出弥漫性细胞内定位。GLUT1和GLUT8在人子宫内膜和子宫内膜癌中均有表达。随着肿瘤组织病理学恶化，GLUT1和GLUT8表达似乎呈逐步进展。GLUT1和GLUT8可能是肿瘤分化的重要标志物，同时也为快速分裂的肿瘤细胞提供能量。
