Department of Biomolecular Science, Division of Toxicology, Hygienic and Environmental Science, University of Urbino Carlo Bo, via S. Chiara 27, Urbino, Italy.
Curr Microbiol. 2012 Apr;64(4):371-8. doi: 10.1007/s00284-012-0080-0. Epub 2012 Jan 22.
The aim of this research was to determine the potential probiotic activity of Lactobacillus acidophilus ATCC 4356 against several human Campylobacter jejuni isolates. The ability to inhibit the pathogen's growth was evaluated by co-culture experiments as well as by antimicrobial assays with cell-free culture supernatant (CFCS), while interference with adhesion/invasion to intestinal Caco-2 cells was studied by exclusion, competition, and displacement tests. In the co-culture experiments L. acidophilus ATCC 4356 strain reduced the growth of C. jejuni with variable percentages of inhibition related to the contact time. The CFCS showed inhibitory activity against C. jejuni strains, stability to low pH, and thermal treatment and sensitivity to proteinase K and trypsin. L. acidophilus ATCC 4356 was able to reduce the adhesion and invasion to Caco-2 cells by most of the human C. jejuni strains. Displacement and exclusion mechanisms seem to be the preferred modalities, which caused a significant reduction of adhesion/invasion of pathogens to intestinal cells. The observed inhibitory properties of L. acidophilus ATCC 4356 on growth ability and on cells adhesion/invasion of C. jejuni may offer potential use of this strain for the management of Campylobacter infections.
本研究旨在确定嗜酸乳杆菌 ATCC 4356 对几种人空肠弯曲菌分离株的潜在益生菌活性。通过共培养实验以及细胞游离培养上清液（CFCS）的抗菌试验评估其抑制病原体生长的能力，同时通过排除、竞争和置换试验研究其对黏附/侵袭肠道 Caco-2 细胞的干扰作用。在共培养实验中，嗜酸乳杆菌 ATCC 4356 菌株以与接触时间相关的可变抑制百分比降低了空肠弯曲菌的生长。CFCS 对空肠弯曲菌菌株表现出抑制活性，对低 pH 值、热处理敏感，对蛋白酶 K 和胰蛋白酶敏感。嗜酸乳杆菌 ATCC 4356 能够降低大多数人源空肠弯曲菌对 Caco-2 细胞的黏附和侵袭。置换和排除机制似乎是首选方式，这导致病原体对肠道细胞的黏附和侵袭显著减少。观察到嗜酸乳杆菌 ATCC 4356 对空肠弯曲菌生长能力和细胞黏附/侵袭的抑制特性可能为该菌株在弯曲菌感染的管理中的潜在用途提供了依据。
