Department of Food Science and Technology, Pukyong National University, Busan 608-737, South Korea.
J Sci Food Agric. 2012 Aug 15;92(10):2084-90. doi: 10.1002/jsfa.5585. Epub 2012 Jan 23.
In an effort to develop alternative therapeutic agents, strong inhibitory activity against α-glucosidase and α-amylase was detected in Eisenia bicyclis methanolic extract.
In this study, two phlorotannins were isolated from E. bicyclis and characterised by chromatography and nuclear magnetic resonance. The active substances were identified as fucofuroeckol A (FF) and dioxinodehydroeckol (DD). To the authors' knowledge, this is the first report of the identification of these substances in E. bicyclis. However, to date, no antidiabetic activity of FF and DD has been reported. Both phlorotannins demonstrated significant inhibitory activity against α-glucosidase and α-amylase. FF showed potent antidiabetic activity, with IC(50) values of 131.34 nmol L(-1) against α-glucosidase and 42.91 µmol L(-1) against α-amylase. The corresponding IC(50) values of DD were 93.33 nmol L(-1) and 472.7 µmol L(-1) . Furthermore, kinetic analysis revealed that FF and DD exhibited non-competitive inhibitory activity against α-glucosidase.
These results suggest that FF and DD may be candidates for the development of an antidiabetic pharmaceutical agent or food additive.
为了开发替代治疗剂，在双齿围沙蚕的甲醇提取物中检测到对α-葡萄糖苷酶和α-淀粉酶有很强的抑制活性。
在这项研究中，从双齿围沙蚕中分离出两种岩藻多酚，并通过色谱和核磁共振进行了表征。活性物质被鉴定为岩藻酚 A（FF）和二氧代去甲环糊精（DD）。据作者所知，这是首次在双齿围沙蚕中鉴定出这些物质。然而，迄今为止，尚未报道 FF 和 DD 具有抗糖尿病活性。两种岩藻多酚均对α-葡萄糖苷酶和α-淀粉酶表现出显著的抑制活性。FF 对α-葡萄糖苷酶表现出很强的抗糖尿病活性，IC（50）值为 131.34 nmol L（-1），对α-淀粉酶的 IC（50）值为 42.91 µmol L（-1）。DD 的相应 IC（50）值分别为 93.33 nmol L（-1）和 472.7 µmol L（-1）。此外，动力学分析表明，FF 和 DD 对α-葡萄糖苷酶表现出非竞争性抑制活性。
这些结果表明，FF 和 DD 可能是开发抗糖尿病药物或食品添加剂的候选物。
