Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand.
Department of Chemical Engineering, Faculty of Engineering, Kasetsart University, Bangkok 10900, Thailand.
Nutrients. 2024 Sep 6;16(17):3017. doi: 10.3390/nu16173017.
Exceeding a healthy weight significantly elevates the likelihood of developing type 2 diabetes (T2DM). A commercially available singular constituent, available as either purified vitexin or iso-vitexin, has been associated with a decreased risk of T2DM, but its synergistic effect has not been reported yet. Vitexin and iso-vitexin were extracted using an ethanol-based solvent from mung bean seed coat (MBCE) and subsequently purified using preparative liquid chromatography (Prep-LC). Eleven mixture ratios of vitexin and/or iso-vitexin were determined for their antioxidant and antihyperglycemic activities. The 1:1.5 ratio of vitexin to iso-vitexin from MBCE demonstrated the most synergistic effects for enzyme inhibition and glucose uptake in HepG2 cells within an insulin-resistant system, while these ratios exhibited a significantly lower antioxidant capacity than that of each individual component. In a gut model system, the ratio of 1:1.5 (vitexin and iso-vitexin) regulated the gut microbiota composition in overweight individuals by decreasing the growth of and , while increasing in and . The application of vitexin/iso-vitexin for 24 h fermentation enhanced a high variety of abundances of 21 genera resulting in five genera of , , , , and , which belonged to the phylum , exhibiting high abundant changes of more than 5%. Only two genera of Proteus and belonging to and decreased. The findings suggest that these phytochemicals interactions could have synergistic effects in regulating glycemia, through changes in antihyperglycemic activity and in the gut microbiota in overweight individuals. This optimal ratio can be utilized by industries to formulate more potent functional ingredients for functional foods and to create nutraceutical supplements aimed at reducing the risk of T2DM in overweight individuals.
体重超标显著增加了 2 型糖尿病（T2DM）的发病风险。一种市售的单一成分，以纯化的牡荆素或异牡荆素的形式存在，与降低 T2DM 的风险有关，但尚未报道其协同作用。从绿豆种皮（MBCE）中使用基于乙醇的溶剂提取牡荆素和异牡荆素，然后使用制备液相色谱（Prep-LC）进行纯化。确定了牡荆素和/或异牡荆素的 11 种混合物比例，以评估其抗氧化和抗高血糖活性。MBCE 中牡荆素与异牡荆素的 1:1.5 比例在胰岛素抵抗系统中对 HepG2 细胞的酶抑制和葡萄糖摄取显示出最协同的作用，而这些比例的抗氧化能力明显低于每个单独成分。在肠道模型系统中，1:1.5（牡荆素和异牡荆素）的比例通过减少 和 的生长，同时增加 和 的生长，调节超重个体的肠道微生物群组成。将牡荆素/异牡荆素应用于 24 小时发酵增强了 21 个属的高多样性丰度，导致 、 、 、 和 属增加，属于门 ，表现出超过 5%的高丰富度变化。只有两个属的变形菌和 属于 和 减少。研究结果表明，这些植物化学物质的相互作用可能通过改变抗高血糖活性和超重个体的肠道微生物群，在调节血糖方面具有协同作用。该最佳比例可被工业界用于开发更有效的功能性成分，用于功能性食品，并创造营养保健品，以降低超重个体患 T2DM 的风险。
