Morita Reiko, Jin Meilan, Ogawa Bun-Ichiro, Kuwata Kazunori, Shibutani Makoto, Mitsumori Kunitoshi
J Toxicol Pathol. 2010 Jun;23(2):95-8. doi: 10.1293/tox.23.95. Epub 2010 Jun 30.
A 10-year-old female miniature poodle had a mass in its carpal joint of the left forelimb. The tumor was divided into small multiple lobules by delicate connective tissues, and necroses were found in some of the central lobules. In some connective stromal areas, chondroid and osteoid tissues were formed. The tumor cells were similar to the structure of apocrine gland epithelial cells with apical blebs resembling apocrine secretion and eosinophilic secretary materials within the luminal space, and spindle cells were sometimes found in the basal area of the glandular structure. In some areas, tumor cells invaded in the blood vessels, bone and bone marrow. Immunohistochemically, the tumor cells forming tubulo-acinar to solid structures were intensely positive for cytokeratin and keratin K8/K18, and the spindle cells were positive for vimentin and alpha-smooth muscle actin. This case was diagnosed as a malignant mixed apocrine gland tumor with metastases to the bone and bone marrow.
一只10岁雌性迷你贵宾犬左前肢腕关节处有一个肿块。肿瘤被纤细的结缔组织分隔成多个小结节，部分中央结节出现坏死。在一些结缔组织基质区域，形成了软骨样和骨样组织。肿瘤细胞类似于顶泌汗腺上皮细胞结构，具有类似顶泌分泌的顶端小泡以及管腔内的嗜酸性分泌物质，在腺结构的基底区域有时可见梭形细胞。在一些区域，肿瘤细胞侵入血管、骨骼和骨髓。免疫组织化学显示，形成管状腺泡至实体结构的肿瘤细胞细胞角蛋白和角蛋白K8/K18呈强阳性，梭形细胞波形蛋白和α-平滑肌肌动蛋白呈阳性。该病例被诊断为伴有骨和骨髓转移的恶性混合性顶泌汗腺肿瘤。
