Zwiers A, Crusius B, Pals G, Donker A J, Meuwissen S G, ten Kate R W
Department of Internal Medicine, Free University Hospital, Amsterdam, The Netherlands.
Gastroenterology. 1990 Dec;99(6):1576-80. doi: 10.1016/0016-5085(90)90460-i.
The pepsinogen A isozymogen pattern in gastric mucosa is genetically determined and can be visualized in nondenaturating polyacrylamide gel electrophoresis of supernatants of sonified gastric mucosal biopsies by demonstrating proteolytic activity after converting pepsinogen into pepsin by acid. Pepsinogen isozymogens are present in very low concentrations in the blood but can now be demonstrated in serum by a newly developed immunoblotting procedure. This study investigated whether the serum pepsinogen A isozymogen pattern adequately reflects the pepsinogen A phenotype. Serum and gastric mucosal pepsinogen A isozymogen patterns were compared in 72 subjects from the routine endoscopy program. A close correlation was found between the relative intensities of the pepsinogen A isozymogens in the serum and the gastric mucosal patterns. Increasing the pepsinogen A release into the circulation by oral omeprazole did not affect the pepsinogen A patterns in the blood. It is concluded that the serum pepsinogen A pattern reflects the pepsinogen A phenotype in humans. In addition, no preferential release of a pepsinogen A isozymogen into the circulation was observed. Thus, immunoblotting of serum provides a new and reliable tool to study pepsinogen genetics in humans. Because a relationship was previously shown between specific pepsinogen A phenotypes and gastric malignancies in humans, serum pepsinogen A patterns may provide a tool to detect subjects who are at risk of gastric cancers.
胃黏膜中的胃蛋白酶原A同工酶模式由基因决定，通过对超声处理后的胃黏膜活检组织上清液进行非变性聚丙烯酰胺凝胶电泳，并在将胃蛋白酶原经酸转化为胃蛋白酶后显示蛋白水解活性，可观察到该模式。胃蛋白酶原同工酶在血液中的浓度极低，但现在可通过一种新开发的免疫印迹法在血清中检测到。本研究调查了血清胃蛋白酶原A同工酶模式是否能充分反映胃蛋白酶原A表型。对常规内镜检查项目中的72名受试者的血清和胃黏膜胃蛋白酶原A同工酶模式进行了比较。发现血清和胃黏膜中胃蛋白酶原A同工酶的相对强度之间存在密切相关性。口服奥美拉唑增加胃蛋白酶原A释放到循环中并未影响血液中的胃蛋白酶原A模式。得出的结论是，血清胃蛋白酶原A模式反映了人类的胃蛋白酶原A表型。此外，未观察到胃蛋白酶原A同工酶向循环中的优先释放。因此，血清免疫印迹为研究人类胃蛋白酶原遗传学提供了一种新的可靠工具。由于先前已表明特定的胃蛋白酶原A表型与人类胃癌之间存在关联，血清胃蛋白酶原A模式可能为检测有患胃癌风险的受试者提供一种工具。
