Zwiers A, Toonstra C, Pals G, Donker A J, Meuwissen S G, ten Kate R W
Department of Internal Medicine, Free University Hospital, Amsterdam, The Netherlands.
J Clin Pathol. 1990 Oct;43(10):871-4. doi: 10.1136/jcp.43.10.871.
Pepsinogen A (PGA) isozymogen patterns in urine and gastric mucosa can be visualised in non-denatured polyacrylamide gel electrophoresis by showing proteolytic activity after the conversion of pepsinogen into pepsin by acid. This method is not suitable for visualising PGA patterns in serum due to low PGA concentrations. To obtain a more sensitive visualisation method an immunoblotting technique was developed. PGA isozymogen patterns from urine and sonified gastric mucosa specimens obtained by immunoblotting were identical with those obtained by activity staining. The immunostaining method was at least 50 times more sensitive. PGA isozymogen patterns could be visualised in serum. Preliminary results suggest that the PGA patterns in serum and gastric mucosa are identical. As an association has been found between the genetically determined PGA isozymogen patterns in gastric mucosa and gastric malignancies in man, immunoblotting of PGA isozymogens in serum may provide a screening tool for subjects at risk of malignant gastric disease.
胃蛋白酶原A（PGA）同工酶原在尿液和胃黏膜中的模式可通过非变性聚丙烯酰胺凝胶电泳进行可视化，方法是在酸将胃蛋白酶原转化为胃蛋白酶后显示蛋白水解活性。由于血清中PGA浓度较低，该方法不适用于可视化血清中的PGA模式。为了获得更灵敏的可视化方法，开发了一种免疫印迹技术。通过免疫印迹获得的尿液和超声处理后的胃黏膜标本中的PGA同工酶原模式与通过活性染色获得的模式相同。免疫染色方法的灵敏度至少高50倍。血清中的PGA同工酶原模式可以可视化。初步结果表明，血清和胃黏膜中的PGA模式相同。由于在人类胃黏膜中遗传决定的PGA同工酶原模式与胃恶性肿瘤之间已发现关联，血清中PGA同工酶原的免疫印迹可能为有患胃恶性疾病风险的受试者提供一种筛查工具。
