Amin Amr, Mahmoud-Ghoneim Doaa, Syam Muhammed I, Daoud Sayel
Biology Department, UAE University, University St, Al-Ain 17551, UAE.
Theor Biol Med Model. 2012 Jan 24;9:1. doi: 10.1186/1742-4682-9-1.
The aim of this study is to assess the protective effects of Ginkgo biloba's (GB) extract against chemotherapeutic-induced reproductive toxicity using a data mining tool, namely Neural Network Clustering (NNC) on two types of data: biochemical & fertility indicators and Texture Analysis (TA) parameters. GB extract (1 g/kg/day) was given orally to male albino rats for 26 days. This period began 21 days before a single cisplatin (CIS) intraperitoneal injection (10 mg/kg body weight). GB given orally significantly restored reproductive function. Tested extract also notably reduced the CIS-induced reproductive toxicity, as evidenced by restoring normal morphology of testes. In GB, the attenuation of CIS-induced damage was associated with less apoptotic cell death both in the testicular tissue and in the sperms. CIS-induced alterations of testicular lipid peroxidation were markedly improved by the examined plant extract. NNC has been used for classifying animal groups based on the quantified biochemical & fertility indicators and microscopic image texture parameters extracted by TA. NNC showed the separation of two clusters and the distribution of groups among them in a way that signifies the dose-dependent protective effect of GB. The present study introduces the neural network as a powerful tool to assess both biochemical and histopathological data. We also show here that herbal protection against CIS-induced reproductive toxicity utilizing classic methodologies is validated using neural network analysis.
本研究旨在使用一种数据挖掘工具，即神经网络聚类（NNC），对生化与生育指标以及纹理分析（TA）参数这两类数据进行分析，以评估银杏叶提取物（GB）对化疗诱导的生殖毒性的保护作用。将GB提取物（1克/千克/天）口服给予雄性白化大鼠，持续26天。这个阶段在单次腹腔注射顺铂（CIS，10毫克/千克体重）前21天开始。口服GB能显著恢复生殖功能。经测试的提取物还显著降低了CIS诱导的生殖毒性，睾丸正常形态的恢复证明了这一点。在GB组中，CIS诱导的损伤减轻与睾丸组织和精子中凋亡细胞死亡减少有关。所检测的植物提取物显著改善了CIS诱导的睾丸脂质过氧化改变。NNC已被用于根据通过TA提取的量化生化与生育指标以及微观图像纹理参数对动物组进行分类。NNC显示出两个聚类的分离以及各组在其中的分布情况，这表明了GB的剂量依赖性保护作用。本研究引入神经网络作为评估生化和组织病理学数据的有力工具。我们在此还表明，利用经典方法对GB预防CIS诱导的生殖毒性的作用通过神经网络分析得到了验证。
