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利用含有guaB - xse和guaB - upp缺失的菌株确定大肠杆菌K - 12鸟嘌呤操纵子的方向。

Orientation of the guanine operon of Escherichia coli K-12 by utilizing strains containing guaB-xse and guaB-upp deletions.

作者信息

Vales L D, Chase J W, Murphy J B

出版信息

J Bacteriol. 1979 Jul;139(1):320-2. doi: 10.1128/jb.139.1.320-322.1979.

Abstract

Temperature induction of an Escherichia coli strains with lambda cI1857 integrated in the guaB gene has been used to produce strains containing chromosomal deletions extending into the xse and upp genes. By utilizing strains containing these deletions, it has been possible to order the genes in the guanine operon with respect to the xseA and upp genes. The order of the genes in this region is glyA-hisS-xseA-guaO-guaB-guaA-purG-upp-purC.

摘要

通过温度诱导整合有λcI1857且位于guaB基因中的大肠杆菌菌株,已成功构建出染色体缺失延伸至xse和upp基因的菌株。利用这些缺失菌株,得以确定鸟嘌呤操纵子中各基因相对于xseA和upp基因的顺序。该区域基因顺序为glyA - hisS - xseA - guaO - guaB - guaA - purG - upp - purC。

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