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利用含有guaB - xse和guaB - upp缺失的菌株确定大肠杆菌K - 12鸟嘌呤操纵子的方向。

Orientation of the guanine operon of Escherichia coli K-12 by utilizing strains containing guaB-xse and guaB-upp deletions.

作者信息

Vales L D, Chase J W, Murphy J B

出版信息

J Bacteriol. 1979 Jul;139(1):320-2. doi: 10.1128/jb.139.1.320-322.1979.

DOI:10.1128/jb.139.1.320-322.1979
PMID:222730
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC216865/
Abstract

Temperature induction of an Escherichia coli strains with lambda cI1857 integrated in the guaB gene has been used to produce strains containing chromosomal deletions extending into the xse and upp genes. By utilizing strains containing these deletions, it has been possible to order the genes in the guanine operon with respect to the xseA and upp genes. The order of the genes in this region is glyA-hisS-xseA-guaO-guaB-guaA-purG-upp-purC.

摘要

通过温度诱导整合有λcI1857且位于guaB基因中的大肠杆菌菌株，已成功构建出染色体缺失延伸至xse和upp基因的菌株。利用这些缺失菌株，得以确定鸟嘌呤操纵子中各基因相对于xseA和upp基因的顺序。该区域基因顺序为glyA - hisS - xseA - guaO - guaB - guaA - purG - upp - purC。

相似文献

1
Orientation of the guanine operon of Escherichia coli K-12 by utilizing strains containing guaB-xse and guaB-upp deletions.利用含有guaB - xse和guaB - upp缺失的菌株确定大肠杆菌K - 12鸟嘌呤操纵子的方向。
J Bacteriol. 1979 Jul;139(1):320-2. doi: 10.1128/jb.139.1.320-322.1979.
2
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J Bacteriol. 1973 Sep;115(3):992-1002. doi: 10.1128/jb.115.3.992-1002.1973.
3
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本文引用的文献

1
Acetylornithinase of Escherichia coli: partial purification and some properties.大肠杆菌的乙酰鸟氨酸酶：部分纯化及某些性质
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2
The gua operon of Escherichia coli K-12: evidence for polarity from guaB to guaA.大肠杆菌K-12的gua操纵子：guaB到guaA存在极性的证据。
J Bacteriol. 1973 Sep;115(3):992-1002. doi: 10.1128/jb.115.3.992-1002.1973.
3
Recalibrated linkage map of Escherichia coli K-12.大肠杆菌K-12的重新校准连锁图谱。
Bacteriol Rev. 1976 Mar;40(1):116-67. doi: 10.1128/br.40.1.116-167.1976.
4
Mapping hisS, the structural gene for histidyl-transfer ribonucleic acid synthetase, in Escherichia coli.在大肠杆菌中定位组氨酰 - 转移核糖核酸合成酶的结构基因hisS。
J Bacteriol. 1979 Apr;138(1):264-7. doi: 10.1128/jb.138.1.264-267.1979.
5
Escherichia coli mutants deficient in exonuclease VII.缺乏核酸外切酶VII的大肠杆菌突变体。
J Bacteriol. 1977 Feb;129(2):934-47. doi: 10.1128/jb.129.2.934-947.1977.