缺乏核酸外切酶VII的大肠杆菌突变体的分离及初步鉴定
Isolation and preliminary characterization of Escherichia coli mutants deficient in exonuclease VII.
作者信息
Vales L D, Rabin B A, Chase J W
出版信息
J Bacteriol. 1983 Sep;155(3):1116-22. doi: 10.1128/jb.155.3.1116-1122.1983.
Abstract
Strains of Escherichia coli containing reduced levels of exonuclease VII activity due to mutations in the xseB gene have been isolated after mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. Seven mutants of independent origin deficient in exonuclease VII activity were obtained. Four of these contained defects in xseA, a locus which has been previously identified, and three others contained mutations in a gene distinct from xseA, which we have designated xseB. Genetic mapping studies place the xseB locus between proC and dnaZ. Exonuclease VII purified from KLC835 (xseA+ xseB3) is more heat labile than enzyme purified from the parent strain PA610 (xse+), showing that xseB is a structural gene for exonuclease VII. The isolation of lambda transducing phage carrying xseA is also described.
摘要
在用N-甲基-N'-硝基-N-亚硝基胍诱变后，已分离出因xseB基因突变而导致核酸外切酶VII活性水平降低的大肠杆菌菌株。获得了七个独立起源的缺乏核酸外切酶VII活性的突变体。其中四个在xseA中有缺陷，xseA是一个先前已鉴定的基因座，另外三个在一个与xseA不同的基因中有突变，我们将其命名为xseB。遗传图谱研究表明xseB基因座位于proC和dnaZ之间。从KLC835（xseA+ xseB3）纯化的核酸外切酶VII比从亲本菌株PA610（xse+）纯化的酶更不耐热，这表明xseB是核酸外切酶VII的结构基因。还描述了携带xseA的λ转导噬菌体的分离。
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