Department of Chemistry/Biochemistry, Philipps-University, Hans-Meerwein-Strasse, D-35032 Marburg, Germany.
FEBS Lett. 2012 Jul 16;586(15):2065-75. doi: 10.1016/j.febslet.2012.01.017. Epub 2012 Jan 21.
Peptide natural products continue to play an important role in modern medicine as last-resort treatments of many life-threatening diseases, as they display many interesting biological activities ranging from antibiotic to antineoplastic. A large fraction of these microbial natural products is assembled by ribosome-independent mechanisms. Progress in sequencing technology and the mechanistic understanding of secondary metabolite pathways has led to the discovery of many formerly cryptic natural products and a molecular understanding of their assembly. Those advances enable us to apply protein and metabolic engineering approaches towards the manipulation of biosynthetic pathways. In this review we discuss the application potential of both templated and non-templated pathways as well as chemoenzymatic strategies for the structural diversification and tailoring of peptide natural products.
肽类天然产物作为许多危及生命疾病的最后治疗手段，继续在现代医学中发挥重要作用，因为它们具有许多有趣的生物活性，从抗生素到抗肿瘤。这些微生物天然产物的很大一部分是通过核糖体独立的机制组装的。测序技术的进步和对次生代谢途径的机制理解导致了许多以前隐藏的天然产物的发现，并对其组装有了分子上的理解。这些进展使我们能够应用蛋白质和代谢工程方法来操纵生物合成途径。在这篇综述中，我们讨论了模板和非模板途径以及化学酶策略在肽类天然产物的结构多样化和修饰方面的应用潜力。
