Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, Japan.
Jpn J Infect Dis. 2012;65(1):33-6.
The utility of active surveillance cultures (ASCs) in respiratory wards, that do not have an associated intensive care unit (ICU), and the usefulness of the BD GeneOhm MRSA™ system for rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) have not been previously evaluated in Japan. ASCs using conventional culture methods and the BD GeneOhm MRSA™ assay were conducted in adult inpatients between May 11, 2009 and November 10, 2009 in a respiratory ward, without an associated ICU, in Nagasaki University Hospital. The infection and colonization rates of MRSA acquired in this respiratory ward were both investigated. A total of 159 patients were investigated. Of these, 12 (7.5%) were found positive for MRSA by the BD GeneOhm MRSA™ assay and 9 (5.7%) were found positive by a conventional culture test upon admission. All cases were MRSA-colonized cases and cross-transmission was not found to occur during hospitalization. The BD GeneOhm MRSA™ assay had a sensitivity of 100% and a specificity of 98%. ASCs in our respiratory ward revealed that MRSA was brought in from other sites in some cases, and that current infection control measures in Nagasaki University Hospital are effective. The BD GeneOhm MRSA™ assay was proven to be a useful and rapid detection tool for MRSA.
在日本，尚未评估不设重症监护病房（ICU）的呼吸病房主动监测培养（ASCs）的实用性，以及 BD GeneOhm MRSA™系统快速检测耐甲氧西林金黄色葡萄球菌（MRSA）的有用性。2009 年 5 月 11 日至 11 月 10 日，在长崎大学医院的一个呼吸病房，对没有相关 ICU 的成年住院患者进行了使用常规培养方法和 BD GeneOhm MRSA™检测的 ASC。对该呼吸病房获得的 MRSA 的感染和定植率进行了调查。共调查了 159 例患者。其中，12 例（7.5%）通过 BD GeneOhm MRSA™检测呈 MRSA 阳性，9 例（5.7%）入院时常规培养检测呈阳性。所有病例均为 MRSA 定植病例，住院期间未发现交叉传播。BD GeneOhm MRSA™检测的敏感性为 100%，特异性为 98%。我们呼吸病房的 ASC 表明，MRSA 是从其他部位带入的，长崎大学医院目前的感染控制措施是有效的。BD GeneOhm MRSA™检测被证明是一种有用且快速的 MRSA 检测工具。
