生物样本的无标记二次谐波产生全息显微镜术

Label-free second harmonic generation holographic microscopy of biological specimens.

作者信息

Masihzadeh Omid, Schlup Philip, Bartels Randy A

机构信息

Department of Electrical and Computer Engineering, Department of Chemistry,and the School of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA.

出版信息

Opt Express. 2010 May 10;18(10):9840-51. doi: 10.1364/OE.18.009840.

DOI:10.1364/OE.18.009840

PMID:20588833

Abstract

Second-order nonlinear holographic microscopy for high-speed, three-dimensional imaging is demonstrated. The use of harmonic generation allows image formation of endogenous features in biological samples such as muscle tissue. We have acquired holograms with acquisition times as short as 10 ms, limited by the switching speed of our shutter; frame rates of 100's of Hz are expected to be possible. The samples are imaged with a Yb:KGW femtosecond laser oscillator, whose 1027 nm wavelength is well suited to minimize absorption and scattering. The low average power of the oscillator prevents damage to the sample.

摘要

展示了用于高速三维成像的二阶非线性全息显微镜。利用谐波产生可对生物样本（如肌肉组织）中的内源性特征进行成像。我们已获取了采集时间短至10毫秒的全息图，这受限于快门的切换速度；预计有可能实现数百赫兹的帧率。使用Yb:KGW飞秒激光振荡器对样本进行成像，其1027纳米波长非常适合将吸收和散射降至最低。该振荡器的低平均功率可防止对样本造成损伤。