The New Zealand Institute for Plant and Food Research Ltd., Auckland, New Zealand.
Arch Virol. 2012 Apr;157(4):713-22. doi: 10.1007/s00705-011-1219-1. Epub 2012 Jan 25.
Two co-infecting novel vitiviruses from Actinidia chinensis were identified from mechanically inoculated Nicotiana occidentalis. Both virus genomes were sequenced and share 64% nucleotide identity. Their overall structure is typical of vitiviruses, with five open reading frames (ORFs) and a polyadenylated 3' end. Open reading frame 4 (ORF4) encodes the coat protein, the most conserved gene of the vitiviruses, in which they share 75% amino acid identity, 61-68% with grapevine virus B, 55-59% with grapevine virus A, and 37-42% with grapevine virus E. Based on the molecular criteria for species demarcation in the family Betaflexiviridae, these are two novel viruses, tentatively named Actinidia virus A and Actinidia virus B.
从经机械接种的黄花烟中鉴定出两种感染猕猴桃的新型番茄斑萎病毒。两种病毒基因组均已测序，其核苷酸序列相似度为 64%。它们的整体结构是典型的番茄斑萎病毒，具有五个开放阅读框（ORF）和一个多聚腺苷酸化的 3'末端。开放阅读框 4（ORF4）编码外壳蛋白，这是番茄斑萎病毒最保守的基因，它们的氨基酸序列相似度为 75%，与葡萄卷叶病毒 B 相似度为 61-68%，与葡萄褪绿病毒 A 相似度为 55-59%，与葡萄卷叶病毒 E 相似度为 37-42%。根据贝塔花束病毒科种的分子界定标准，这两种病毒是两种新型病毒，暂定名为猕猴桃病毒 A 和猕猴桃病毒 B。
