细胞培养适应的韩国人 G9P[8]轮状病毒，CAU05-202 的基因组特征。

Genomic characterization of a cell-culture-adapted Korean human G9P[8] rotavirus, CAU05-202.

机构信息

Department of Internal Medicine, College of Medicine, Chung-Ang University, Seoul, South Korea.

出版信息

Arch Virol. 2012 Apr;157(4):753-9. doi: 10.1007/s00705-012-1232-z. Epub 2012 Jan 25.

Abstract

The human rotavirus G9 strain is the fifth most common rotavirus worldwide. A human rotavirus G9P[8] strain CAU05-202 was isolated from a young child with diarrhea using a cell culture system, and its major gene sequences were determined. Phylogenetic analysis of the VP7 gene revealed that CAU05-202 clustered into genetic lineage III-d and was most closely related to G9 rotaviruses from Turkey (strain GUH13) and Sri Lanka (strain 05SLC056 and 05SLC057). VP4 and NSP4 gene analysis showed that CAU05-202 belongs to the P[8]-3 lineage and genotype B, respectively. In addition, CAU05-202 has a long RNA electropherotype, supported by VP6 gene analysis, which is clearly associated with subgroup II specificity. Analysis of the G9 rotavirus strain CAU05-202 provides information concerning the genetic relationships among global rotavirus G9 strains, suggesting that closely related G9 strains are persistent and widespread in Asian countries.

摘要

人轮状病毒 G9 株是世界上第五种最常见的轮状病毒。一株人轮状病毒 G9P[8]CAU05-202 株是从一名腹泻的幼儿中利用细胞培养系统分离得到的，并对其主要基因序列进行了测定。VP7 基因的系统进化分析表明，CAU05-202 聚类于基因谱系 III-d，与来自土耳其（GUH13 株）和斯里兰卡（05SLC056 株和 05SLC057 株）的 G9 轮状病毒最为密切相关。VP4 和 NSP4 基因分析表明，CAU05-202 分别属于 P[8]-3 谱系和基因型 B。此外，CAU05-202 具有长的 RNA 电泳型，VP6 基因分析支持这一点，这与亚组 II 的特异性明显相关。对 CAU05-202 株人轮状病毒 G9 株的分析提供了有关全球轮状病毒 G9 株之间遗传关系的信息，表明密切相关的 G9 株在亚洲国家具有持续性和广泛存在性。