Conservation Biology and Molecular Taxonomy Laboratory, CSIR-National Botanical Research Institute Rana Pratap Marg, Lucknow 226001, India.
Mol Biotechnol. 2013 Feb;53(2):139-49. doi: 10.1007/s12033-012-9500-4.
Murraya koenigii (L.) Spreng. (Rutaceae), is an aromatic plant and much valued for its flavor, nutritive and medicinal properties. In this study, three DNA fingerprinting methods viz., random amplification of polymorphic DNA (RAPD), directed amplification of minisatellite DNA (DAMD), and inter-simple sequence repeat (ISSR), were used to unravel the genetic variability and relationships across 92 wild and cultivated M. koenigii accessions. A total of 310, 102, and 184, DNA fragments were amplified using 20 RAPD, 5 DAMD, and 13 ISSR primers, revealing 95.80, 96.07, and 96.73% polymorphism, respectively, across all accessions. The average polymorphic information content value obtained with RAPD, DAMD, and ISSR markers was 0.244, 0.250, and 0.281, respectively. The UPGMA tree, based on Jaccard's similarity coefficient generated from the cumulative (RAPD, DAMD, and ISSR) band data showed two distinct clusters, clearly separating wild and cultivated accessions in the dendrogram. Percentage polymorphism, gene diversity (H), and Shannon information index (I) estimates were higher in cultivated accessions compared to wild accessions. The overall high level of polymorphism and varied range of genetic distances revealed a wide genetic base in M. koenigii accessions. The study suggests that RAPD, DAMD, and ISSR markers are highly useful to unravel the genetic variability in wild and cultivated accessions of M. koenigii.
九里香（Murraya koenigii（L.）Spreng.）（芸香科）是一种芳香植物，因其风味、营养和药用特性而备受珍视。在这项研究中，使用了三种 DNA 指纹图谱方法，即随机扩增多态性 DNA（RAPD）、微卫星定向扩增（DAMD）和简单重复间序列（ISSR），以揭示 92 个野生和栽培九里香品种的遗传变异性和关系。使用 20 个 RAPD、5 个 DAMD 和 13 个 ISSR 引物共扩增了 310、102 和 184 个 DNA 片段，分别显示所有品种的 95.80%、96.07%和 96.73%的多态性。RAPD、DAMD 和 ISSR 标记获得的平均多态信息含量值分别为 0.244、0.250 和 0.281。基于累积（RAPD、DAMD 和 ISSR）带数据的 Jaccard 相似系数生成的 UPGMA 树显示了两个不同的聚类，在树状图中清楚地分离了野生和栽培品种。与野生品种相比，栽培品种的多态百分率、基因多样性（H）和香农信息指数（I）估计值更高。九里香品种的整体高水平多态性和遗传距离的变化范围表明其具有广泛的遗传基础。研究表明，RAPD、DAMD 和 ISSR 标记非常有助于揭示野生和栽培九里香品种的遗传变异性。
