Tung Yen-Ting, Wu Ming-Fang, Wang Gou-Jen, Hsieh Shie-Liang
Ph.D. Program in Tissue Engineering and Regenerative Medicine, National Chung-Hsing University, Taichung, Taiwan.
Genomics Research Center, Academia Sinica, Taipei, Taiwan.
Nanomedicine. 2014 Aug;10(6):1335-41. doi: 10.1016/j.nano.2014.03.009. Epub 2014 Mar 24.
In this paper, we develop an effective method for detecting weak molecular bonding between the dengue virus (DV) and its receptor C-type lectin domain family 5, member A (CLEC5A). The CLEC5A-DV interaction is critical for DV-induced hemorrhagic fever and shock syndrome, so the sensing of CLEC5A-DV binding is crucial to realize a thorough study of the pathogenesis of dengue fever. Through a highly sensitive nanostructured sensing electrode of gold nanoparticles (GNPs) uniformly deposited on a nanohemisphere array, a label-free detection of the ultra weak binding between CLEC5A and the DV can be performed with electrochemical impedance spectroscopy (EIS). Experimental results demonstrate that the proposed approach is a highly promising method for investigating weak molecular interactions such as the ligand-receptor interaction of dengue fever, enterovirus (EV), or the interaction between cancer surface glycoproteins and their receptors.
Authors of this study investigated the ultra-weak binding between dengue virus and its CLEC5A receptor via electrochemical impedance spectroscopy and gold NP sensing electrode. Similar methods may be applicable in other infections and in cancer models as well.
在本文中，我们开发了一种有效的方法来检测登革热病毒（DV）与其受体C型凝集素结构域家族5成员A（CLEC5A）之间的弱分子键合。CLEC5A-DV相互作用对于DV诱导的出血热和休克综合征至关重要，因此检测CLEC5A-DV结合对于全面研究登革热发病机制至关重要。通过将金纳米颗粒（GNP）均匀沉积在纳米半球阵列上制成的高灵敏度纳米结构传感电极，可利用电化学阻抗谱（EIS）对CLEC5A与DV之间的超弱结合进行无标记检测。实验结果表明，所提出的方法是研究诸如登革热、肠道病毒（EV）的配体-受体相互作用或癌症表面糖蛋白与其受体之间相互作用等弱分子相互作用的极有前景的方法。
本研究的作者通过电化学阻抗谱和金纳米颗粒传感电极研究了登革热病毒与其CLEC5A受体之间的超弱结合。类似的方法也可能适用于其他感染和癌症模型。
