Department of Pathology and Regenerative Medicine, School of Dentistry, Institute for Hard Tissue and Bio-Tooth Regeneration, Kyungpook National University, Daegu, Korea.
J Biomed Mater Res B Appl Biomater. 2012 Apr;100(3):841-9. doi: 10.1002/jbm.b.32648. Epub 2012 Jan 25.
Calcium phosphate ceramics have been widely used as scaffolds for bone regeneration. Here, to improve the osteogenic potential of hydroxyapatite/β-tricalcium phosphate (HA/β-TCP) and to apply the bioactive peptide in situ, matrix extracellular phosphoglycoprotein (MEPE) peptide, which has been shown to stimulate osteoblast differentiation, was covalently and directionally immobilized on HA/β-TCP particles. The free-hydroxyl groups on the surface of the HA/β-TCP particles were sequentially conjugated with APTES, PEG-(SS)(2), and the synthetic MEPE peptide. Using FTIR and XPS, immobilization of the MEPE peptide on the HA/β-TCP was confirmed. Implantation of the MEPE peptide-immobilized HA/β-TCP into calvarial defect and subsequent analyses using a micro CT and histology showed significant bone regeneration and increased bone area (9.89-fold) as compared to that of unmodified HA/β-TCP. Moreover, tartrate-resistant acid phosphatase-positive osteoclasts were observed in regenerated bone by the MEPE peptide-immobilized HA/β-TCP, indicating that the bones newly formed by the MEPE peptide-immobilized HA/β-TCP are actively remodeled by osteoclasts. Therefore, our data demonstrate that MEPE peptide immobilization onto the HA/β-TCP surface stimulates bone regeneration associated with physiological bone remodeling.
磷酸钙陶瓷已被广泛用作骨再生的支架。在这里，为了提高羟基磷灰石/β-磷酸三钙（HA/β-TCP）的成骨潜力，并就地应用生物活性肽，已证明基质细胞外磷蛋白（MEPE）肽能刺激成骨细胞分化，将其共价且定向固定在 HA/β-TCP 颗粒上。HA/β-TCP 颗粒表面的游离羟基依次与 APTES、PEG-(SS)(2)和合成的 MEPE 肽缀合。通过傅里叶变换红外光谱（FTIR）和 X 射线光电子能谱（XPS）证实了 MEPE 肽在 HA/β-TCP 上的固定。将 MEPE 肽固定化的 HA/β-TCP 植入颅骨缺损，并通过微 CT 和组织学进行后续分析表明，与未修饰的 HA/β-TCP 相比，骨再生和骨面积增加（9.89 倍）。此外，在 MEPE 肽固定化的 HA/β-TCP 再生的骨中观察到抗酒石酸酸性磷酸酶阳性破骨细胞，表明 MEPE 肽固定化的 HA/β-TCP 形成的新骨被破骨细胞积极重塑。因此，我们的数据表明，MEPE 肽固定在 HA/β-TCP 表面上刺激与生理骨重塑相关的骨再生。
