塑料表面薄层免疫分析（TIA）中的可视化原理
Visualization principles in thin-layer immunoassays (TIA) on plastic surfaces.
作者信息
Elwing H, Nilsson L, Ouchterlony O
出版信息
Int Arch Allergy Appl Immunol. 1976;51(6):757-62. doi: 10.1159/000231654.
PMID:793997
Abstract
Macromolecules may adsorb firmly as a monolayer to plastic surfaces and still retain their property to react specifically with antibodies. In the present communication four different principles for visualization of such antigenantibody interactions on plastic surfaces are described. In addition, a diffusion-ingel method for quantitation of antibodies is presented. The described methods offer simple, sensitive and accurate means of assaying antigen-antibody reactions.
摘要
大分子物质可以作为单分子层牢固地吸附在塑料表面，并且仍然保留其与抗体特异性反应的特性。在本通讯中，描述了四种在塑料表面可视化此类抗原-抗体相互作用的不同原理。此外，还介绍了一种用于定量抗体的扩散凝胶法。所描述的方法提供了简单、灵敏且准确的抗原-抗体反应检测手段。
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