Lange S, Elwing H, Larsson P, Nygren H
J Clin Microbiol. 1980 Nov;12(5):637-40. doi: 10.1128/jcm.12.5.637-640.1980.
The diffusion-in-gel enzyme-linked immunosorbent assay (DIG-ELISA) is a new and simple method for quantitation of antibodies, based on the ability of antibodies to diffuse from wells in gel and adsorb to antigen which is bound to a polystyrene surface. The antigen-antibody reaction is visualized with a color reaction caused by horseradish peroxidase-conjugated class-specific anti-immunoglobins. This method was used to study the immunoglobulin G, A, and M immune response to Salmonella typhi O antigen in individuals immunized with a monovalent heat-inactivated typhoid vaccine. The antibody values obtained by the DIG-ELISA method correlated with those evaluated by conventional direct agglutination (Widal) and indirect hemagglutination methods. The DIG-ELISA method was also found to be sensitive, specific, and economical, as well as suitable for handling large numbers of sera while requiring very simple equipment.
凝胶扩散酶联免疫吸附测定法（DIG-ELISA）是一种新型的、简单的抗体定量方法，其基于抗体从凝胶孔中扩散并吸附到结合于聚苯乙烯表面的抗原上的能力。抗原-抗体反应通过辣根过氧化物酶偶联的类特异性抗免疫球蛋白引起的颜色反应来显现。该方法用于研究用单价热灭活伤寒疫苗免疫的个体对伤寒沙门氏菌O抗原的免疫球蛋白G、A和M免疫反应。通过DIG-ELISA方法获得的抗体值与通过传统直接凝集（肥达氏反应）和间接血凝方法评估的值相关。还发现DIG-ELISA方法灵敏、特异且经济，适合处理大量血清，同时所需设备非常简单。
