Division of Plant Biotechnology, College of Agriculture & Life Science, Chonnam National University, Buk-gu, Gwangju, Korea.
Curr Drug Targets. 2012 Apr;13(4):442-70. doi: 10.2174/138945012799499721.
Plants utilized for agricultural productions interact with insects, fungi, and bacteria under the field conditions, affecting thereby their productivity. Since chitin and its derivatives play important roles in the interactions between these organisms, chitin-related enzymes are effective tools or drug targets for controlling the interactions. Thus, the molecular biology, protein chemistry, and enzymology of the chitin-related enzymes have been intensively studied by many investigators. Identifications and classifications of the genes encoding chitin synthetases, chitinases, chitosanases, and chitin deacetylases in these organisms were conducted, and their physiological functions were defined by knockdown, knockout, or overexpression of the corresponding genes. Recombinant enzyme productions and mutation studies are also being conducted to understand their structure and function. All of these studies have opened the way to efficiently utilize these enzyme tools for enhancing the agricultural productions.
在田间条件下,用于农业生产的植物与昆虫、真菌和细菌相互作用,从而影响其生产力。由于几丁质及其衍生物在这些生物体之间的相互作用中起着重要作用,因此几丁质相关酶是控制相互作用的有效工具或药物靶点。因此,许多研究人员对几丁质相关酶的分子生物学、蛋白质化学和酶学进行了深入研究。对这些生物体中编码几丁质合成酶、几丁质酶、壳聚糖酶和几丁质脱乙酰酶的基因进行了鉴定和分类,并通过敲低、敲除或过表达相应基因来定义其生理功能。还进行了重组酶生产和突变研究,以了解它们的结构和功能。所有这些研究都为有效地利用这些酶工具来提高农业生产开辟了道路。