来自……的具有抗真菌活性的GH家族20外-β-N-乙酰己糖胺酶的特性分析

Shirasaka Naoki, Harazono Koichi, Nakahigashi Ryota, Mitsui Keigo, Tanaka Jun, Tanazawa Sayaka, Mitsutomi Masaru, Ohnuma Takayuki

1 Nagase ChemteX Corporation.

2 Department of Advanced Bioscience, Kindai University.

J Appl Glycosci (1999). 2019 Aug 20;66(3):83-88. doi: 10.5458/jag.jag.JAG-2019_0001. eCollection 2019.

We characterized HEX, which is a glycoside hydrolase (GH) family 20 exo-β--acetylhexosaminidase found in . HEX exolytically hydrolyzed chitin oligosaccharides from their non-reducing ends, and yielded -acetylglucosamine (GlcNAc) as the end product. According to the initial rate of substrate hydrolysis, the rates of (GlcNAc) and (GlcNAc) hydrolysis were greater than the rates for the other oligosaccharides. The enzyme exhibited antifungal activity against , which was probably due to hydrolytic activity with regard to chitin in the hyphal tips. Therefore, HEX has potential for use in GlcNAc production and food preservation.

我们对己糖胺酶（HEX）进行了表征，它是一种在……中发现的糖苷水解酶（GH）家族20外切-β-N-乙酰己糖胺酶。HEX从其非还原端对外切水解几丁质寡糖，并产生N-乙酰葡糖胺（GlcNAc）作为终产物。根据底物水解的初始速率，（GlcNAc）和（GlcNAc）的水解速率大于其他寡糖的水解速率。该酶对……表现出抗真菌活性，这可能是由于其对菌丝尖端几丁质的水解活性。因此，HEX在GlcNAc生产和食品保鲜方面具有应用潜力。