Department of Biochemistry and Molecular Biology, National Institute of Perinatology Isidro Espinosa de los Reyes, Montes Urales # 800, Col. Lomas de Virreyes cp 11000, Mexico City, Mexico.
Placenta. 2012 Apr;33(4):271-7. doi: 10.1016/j.placenta.2012.01.007. Epub 2012 Jan 24.
Overall, 1-4% of all births in the US are complicated by choriamnionitis. Choriamnionitis is a polymicrobial infection most often due to ascending genital microbes which, in over 65% of positive amniotic fluid cultures, involves two or more organisms. In this study, we determine the cytokines expression (IL-1β, TNFα) and prometalloproteinase activation (proMMP-2 and proMMP-9) after double o single infection an in vitro model of human fetal membranes. Fetal membranes at term were mounted in the Transwell culture system and after 24 h of infection, choriodecidual, and amnion media was collected. IL-1β and TNFα were evaluated by ELISA, whereas proMMP-9 and proMMP-2 were determined by substrate gel zymography. The choriodecidual and amnion compartments actively respond to the infectious process, which induced the secretion of IL-1β, TNFα, and proMMP-9 after either mixed or single infection. The proMMP-2 secretion profile was the same after all experimental conditions. There was no synergy between Streptococcus agalactiae and Escherichia coli for inducing the secretion of inflammatory factors or degradative metalloproteinase. In conclusion, these two bacteria could initiate different pathways to induce chorioamnioitis.
总体而言，美国所有分娩中有 1-4%受到绒毛膜羊膜炎的影响。绒毛膜羊膜炎是一种多微生物感染，最常见的原因是生殖道微生物上行，在超过 65%的阳性羊水培养物中，涉及两种或更多种微生物。在这项研究中，我们确定了人胎膜体外模型中双重或单一感染后细胞因子表达（IL-1β、TNFα）和前金属蛋白酶激活（proMMP-2 和 proMMP-9）。足月胎膜在 Transwell 培养系统中安装，感染 24 小时后收集绒毛膜蜕膜和羊膜培养基。通过 ELISA 评估 IL-1β 和 TNFα，通过基质凝胶电泳法测定 proMMP-9 和 proMMP-2。绒毛膜蜕膜和羊膜腔积极响应感染过程，无论是混合感染还是单一感染，都会诱导 IL-1β、TNFα 和 proMMP-9 的分泌。在所有实验条件下，proMMP-2 的分泌模式相同。无乳链球菌和大肠杆菌之间没有协同作用，不能诱导炎症因子或降解金属蛋白酶的分泌。总之，这两种细菌可以启动不同的途径引发绒毛膜羊膜炎。
