Laboratório de Virologia Veterinária, Faculdade de Veterinária 9090, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
Virus Res. 2012 Apr;165(1):29-33. doi: 10.1016/j.virusres.2012.01.001. Epub 2012 Jan 8.
Detection and characterisation of the canine parvovirus (CPV-2) strains that are currently circulating are essential for the understanding of viral evolution and the development of measures to control its spread. In the present study, stool samples from 144 dogs were analysed by polymerase chain reaction (PCR) for CPV-2, and 29.2% (42/144) of them were positive. From the 42 positive strains, 71.4% (30) of the dogs had signs of haemorrhagic gastroenteritis. The sequencing of the 583 bp fragment of the VP2 gene from the positive strains identified 78.6% (33/42) of them as type 2c, 19% (8/42) as type 2b and 2.4% (1/42) as type 2a. A phylogenetic analysis of the variants circulating in the canine population of Brazil showed that they are very similar to those found in other countries and type 2c has become the predominant type circulating in Brazil.
检测和分析目前流行的犬细小病毒 (CPV-2) 株对于了解病毒进化和制定控制其传播的措施至关重要。在本研究中，通过聚合酶链反应 (PCR) 对 144 只狗的粪便样本进行了 CPV-2 分析，其中 29.2%（42/144）呈阳性。在 42 株阳性株中，71.4%（30 株）的狗出现了出血性胃肠炎的症状。对阳性株的 VP2 基因 583 bp 片段进行测序，确定其中 78.6%（33/42）为 2c 型，19%（8/42）为 2b 型，2.4%（1/42）为 2a 型。对巴西犬群中流行的变异株进行的系统进化分析表明，它们与其他国家发现的变异株非常相似，2c 型已成为巴西流行的主要型别。
