神经节苷脂使无反应性成纤维细胞对大肠杆菌不耐热肠毒素敏感。
Gangliosides sensitize unresponsive fibroblasts to Escherichia coli heat-labile enterotoxin.
作者信息
Moss J, Garrison S, Fishman P H, Richardson S H
出版信息
J Clin Invest. 1979 Aug;64(2):381-4. doi: 10.1172/JCI109472.
Abstract
Chemically transformed mouse fibroblasts did not raise their cyclic AMP level in response to Escherichia coli heat-labile enterotoxin. These fibroblasts did, however, incorporate exogenous mono-, di-, and trisialogangliosides. After the uptake of monosialoganglioside galactosyl-N-acetylgalactosaminyl-[N-acetylneuraminyl]-galactosylglucosylceramide (GM1), the cells responded to E. coli heat-labile enterotoxin. The di- and trisialogangliosides were considerably less effective. GM1, the putative cholera toxin (choleragen) receptor, has been implicated previously as the receptor for E. coli heat-labile enterotoxin based on the ability of the free ganglioside to inhibit the effects of toxin. This investigation establishes that the ganglioside, when incorporated into fibroblasts, serves a functional role in mediating the responsiveness to the toxin.
摘要
化学转化的小鼠成纤维细胞对大肠杆菌不耐热肠毒素无反应，其环磷酸腺苷水平不会升高。然而，这些成纤维细胞能够摄取外源性单唾液酸、二唾液酸和三唾液酸神经节苷脂。摄取单唾液酸神经节苷脂半乳糖基-N-乙酰半乳糖胺基-[N-乙酰神经氨酸基]-半乳糖基葡萄糖神经酰胺（GM1）后，细胞对大肠杆菌不耐热肠毒素产生反应。二唾液酸和三唾液酸神经节苷脂的效果则要差得多。GM1是公认的霍乱毒素受体，此前基于游离神经节苷脂抑制毒素作用的能力，它被认为也是大肠杆菌不耐热肠毒素的受体。本研究证实，当神经节苷脂掺入成纤维细胞时，它在介导细胞对毒素的反应中发挥功能性作用。
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J Clin Invest. 1979 Aug;64(2):381-4. doi: 10.1172/JCI109472.
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Polymyxin B-Induced Release of Low-Molecular-Weight, Heat-Labile Enterotoxin from Escherichia coli.多黏菌素 B 诱导大肠埃希菌释放低分子量热不稳定肠毒素。
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Comparison of the action of Escherichia coli enterotoxin on the thymocyte adenylate cyclase-cyclic adenosine monophosphate system to that of cholera toxin and prostaglandin E1.大肠杆菌肠毒素对胸腺细胞腺苷酸环化酶 - 环磷酸腺苷系统的作用与霍乱毒素和前列腺素E1作用的比较。
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Toxins of Vibrio cholerae and Escherichia coli stimulate adenyl cyclase in rat fat cells.霍乱弧菌和大肠杆菌的毒素可刺激大鼠脂肪细胞中的腺苷酸环化酶。
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Absence of a specific ganglioside galactosyltransferase in mouse cells transformed by murine sarcoma virus.被鼠肉瘤病毒转化的小鼠细胞中缺乏特定的神经节苷脂半乳糖基转移酶。
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Stimulation of intestinal adenyl cyclase by Escherichia coli enterotoxin: comparison of strains from an infant and an adult with diarrhea.大肠杆菌肠毒素对肠道腺苷酸环化酶的刺激作用：来自一名腹泻婴儿和一名腹泻成人的菌株比较。
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