Central Scientific Instruments Organisation, Sector (CSIR-CSIO) 30 C, Chandigarh, India.
Biosens Bioelectron. 2012 Mar 15;33(1):190-5. doi: 10.1016/j.bios.2011.12.051. Epub 2012 Jan 6.
Glucose oxidase (GOD) immobilized long period grating (LPG) fibers have been proposed for the specific and sensitive detection of glucose. The treatment of LPG fibers with aminopropyl triethoxysilane has induced biding sites for the subsequent GOD immobilization. Field emission scanning electron microscopy, confocal laser scanning microscopy, infrared spectroscopy and Raman spectroscopy have provided detailed evidences about the effectiveness of the adopted biofunctionalization methodology. The enzyme activity is conserved during the immobilization step. Fabricated LPG sensor was tested on different glucose solutions to record the transmission spectra on an optical spectrum analyzer. The wavelength shifts in the transmission spectra are linearly correlated with the glucose concentration in the range of 10-300 mg dL(-1). The fabricated sensor gives fast response and is demonstrated to be of practical utility by determining glucose contents in blood samples. Proposed technique can further be extended to develop LPG fiber based novel, sensitive and label free nanosensors for disease diagnosis and clinical analysis.
葡萄糖氧化酶（GOD）固定化长周期光纤光栅（LPG）已被提议用于葡萄糖的特异性和灵敏检测。用氨丙基三乙氧基硅烷处理 LPG 光纤诱导了随后 GOD 固定化的结合位点。场发射扫描电子显微镜、共焦激光扫描显微镜、红外光谱和拉曼光谱提供了关于所采用的生物功能化方法的有效性的详细证据。在固定化步骤中保持了酶的活性。将制备的 LPG 传感器在不同的葡萄糖溶液上进行测试，以在光谱分析仪上记录传输光谱。传输光谱中的波长位移与 10-300mg dL(-1)范围内的葡萄糖浓度呈线性相关。所制备的传感器具有快速的响应，并通过测定血液样本中的葡萄糖含量证明具有实际应用价值。所提出的技术可以进一步扩展，以开发基于 LPG 光纤的新型、灵敏和无标记纳米传感器，用于疾病诊断和临床分析。
